Project description:The Aloe vera transcriptome was analysed by hybridising triplicate samples of root and leaf tissue to the Affymetrix Arabidopsis ATH1 array.
Project description:The Aloe vera transcriptome was analysed by hybridising triplicate samples of root and leaf tissue to the Affymetrix Arabidopsis ATH1 array. In total, 7 samples were hybridised to the array. Samples consisted of 1 genomic DNA, and triplicate samples of leaf and root RNA.
Project description:SWATH-MS data for human keratinocytes (HaCaT cell line), sample 0001-0004 indicates HaCaT cells with no treatment, sample 0005-0008 indicates HaCaT cells induced by H2O2, sample 0009-0012 indicates HaCaT cells treated by H2O2 and OLE. For more detailed information, please contact Dr.Chang Liu (hichang813@uri.edu)
Project description:SWATH-MS data for human keratinocytes (HaCaT cell line), sample 010-012 indicates HaCaT cells with no treatment, sample 013-015 indicates HaCaT cells induced by erastin, sample 0016-018 indicates HaCaT cells treated by erastin and CBD. For more detailed information, please contact Dr.Chang Liu (hichang813@uri.edu)
Project description:We employed human HaCaT cells as a model system to identify cellular proteins that accompany SDS-induced toxicity based on a proteomic approach. HaCaT human keratinocyte cell line were treated with a non-cytotoxic dose of SDS (25 µg/ml, as determined by the MTT assay and microscopically examination) for 48 h. The altered abundance of proteins from HaCaT keratinocytes exposed to SDS was analyzed by LC-MS/MS approach and quantified using Progenesis LC software. The abundance of 217 proteins (which were identified by multiple peptides, ≥ 2) was altered in keratinocytes exposed to SDS; in which 131 proteins had increased abundance while 86 proteins was down regulated. The Pathview map of 131 up-regulated proteins was built and enhancement of glycolysis/gluconeogenesis was found.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
