Project description:Comparing the transcriptomic responses between the Mycobacterium avium subspecies paratuberculosis (MAP) leuD mutant with the parent strain K-10 under different environmental stresses: nutrition, temperature, anaerobic conditions, high- and low- pH conditions.
Project description:Global transcriptional profiles of Saccharomyces cerevisiae were studied following changes in growth conditions to high hydrostatic pressure and low temperature. These profiles were quantitatively very similar, encompassing 561 co-upregulated genes and 161 co-downregulated genes. In particular, expression of the DAN/TIR cell wall mannoprotein genes, which are generally expressed under hypoxia, were markedly upregulated by high pressure and low temperature, suggesting the overlapping regulatory networks of transcription. In support of the role of the mannoproteins in cell wall integrity, cells acquired resistance against treatment with SDS, Zymolyase and lethal level of high pressure when preincubated under high pressure and low temperature. Keywords: stress response
Project description:We performed an in-depth proteome analysis of the haloarchaeal model organism Haloferax volcanii under standard, low and high salt and low and high temperature conditions using label-free mass spectrometry. Qualitative analysis of protein identification data from high pH/reversed phase fractionated samples indicated 61.1% proteome coverage (2,509 proteins), which is close to the maximum recorded values in archaea. Identified proteins matched to the predicted proteome in their physicochemical protein properties, with only a small bias against low molecular wright and membrane-associated proteins. Cells grown under low and high salt stress as well as low and high temperature stress were quantitatively compared to standard cultures by SWATH mass spectrometry. 2,244 proteins, or 54.7% of the predicted proteome, were quantified across all conditions at high reproducibility which allowed for global analysis of protein expression changes under these stresses. Pathway enrichment analysis by KEGG annotation showed that most major cellular pathways are part of H. volcanii’s universal stress response. In addition, specific pathways were found to be selectively affected by either salt or temperature stress.
Project description:To understand expression phenotypes in Arabidopsis thaliana at high temperature, a set of the Multiparent Advanced Generation Inter-Cross (MAGIC) recombinant inbred lines were grown under long day conditions (16 hours light: 8 hours dark) at 20˚C and then shifted to 30˚C for 48 hours. RNA was collected at the 4th true leaf stage, and RNA-seq data was generated using the Illumina method. The MAGIC lines included are largely a subset of lines for which comparable RNA-seq data was collected at a constant 20˚C (allowing the same genotypes to be compared at 20˚C and after the high temperature shift at approximately the same developmental stage and under otherwise the same growth conditions). The read data for the analogous low temperature study can be found under GEO submission GSE94107.
2017-04-22 | GSE96812 | GEO
Project description:Transcriptome data of Gracilariopsis lemaneiformis under low temperature stress
| PRJNA716486 | ENA
Project description:Transcriptome data of elephant grass under low-temperature stress
| PRJNA1007572 | ENA
Project description:pepper seeding under low temperature
| PRJNA1056762 | ENA
Project description:Sweetpotato under low temperature stress
