Project description:We applied Solexa sequencing technology to identify Singapore grouper iridovirus (SGIV) encoded microRNAs during its infection. A small RNA library arising from SGIV infected grouper cells (GP) was constructed and sequenced. We recovered 6,802,977 usable reads, of which 34,400 reads represented the small RNA sequences encoded by SGIV. Among them, 16 novel SGIV encoded miRNAs were identified by a computational pipeline. Generally, these 16 miRNAs are dispersed throughout the SGIV genome, while three of them are located within open reading frame 057L (ORF057L) region. Meanwhile, We identified 138 conserved microRNA genes between grouper fish and zebrafish.
Project description:We applied Solexa sequencing technology to identify Singapore grouper iridovirus (SGIV) encoded microRNAs during its infection. A small RNA library arising from SGIV infected grouper cells (GP) was constructed and sequenced. We recovered 6,802,977 usable reads, of which 34,400 reads represented the small RNA sequences encoded by SGIV. Among them, 16 novel SGIV encoded miRNAs were identified by a computational pipeline. Generally, these 16 miRNAs are dispersed throughout the SGIV genome, while three of them are located within open reading frame 057L (ORF057L) region. Meanwhile, We identified 138 conserved microRNA genes between grouper fish and zebrafish. 18-30 nt small RNAs from SGIV-infected GP cells were sequenced in one Solexa lane.
Project description:To determine the protein sequences of various capsid proteins of Singapore grouper iridovirus (SGIV), the proteomic analysis of the purified SGIV sample was performed.
Project description:this is the raw data accompanying the following publication:
Bromenshenk JJ, Henderson CB, Wick CH, Stanford MF, Zulich AW, Jabbour RE,
Deshpande SV, McCubbin PE, Seccomb RA, Welch PM, Williams T, Firth DR, Skowronski
E, Lehmann MM, Bilimoria SL, Gress J, Wanner KW, Cramer RA Jr. Iridovirus and
microsporidian linked to honey bee colony decline. PLoS One. 2010 Oct
6;5(10):e13181. doi: 10.1371/journal.pone.0013181. PubMed PMID: 20949138; PubMed
Central PMCID: PMC2950847.
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:Singapore grouper iridovirus (SGIV) is the major agent that causes severe iridovirus diseases in grouper maricluture. Based on the genomic information, a DNA microarray, containing probes corresponding to 162 putative SGIV open reading frames (ORFs) was constucted. The viral microarrays wereused to classify the majority of SGIV transcripts into three temporal kinetic classes (immediate-early, IE; early, E; late, L) during an in vitro infection by their dependence on de novo protein synthesis inhibitor and viral DNA replication. Keywords: drug response
Project description:Iridovirus is an important viral pathogen affecting large yellow croaker. Megalocytivirus FD2018 with a spanning genome of 112,214 bp double-stranded DNA with a G + C content of 53.53% and 130 predicted genes with ORF sizes of 41 to 1293 amino acids, these virion proteins are predicted to be involved in virion assembly, DNA replication, transcription, nucleotide metabolism and protein modification. Proteomic analysis identified 55 proteins in the cell culture supernatants and purified viral particles.
