Project description:Parasitic infections with the hookworms Ancylostoma ceylanicum and Necator americanus are a significant global health burden and current hookworm infection management approaches are limited by modest drug efficacy, costs, risk of reinfection and drug resistance. Subunit vaccines based on proteins excreted and secreted (ES) by hookworms that impart sufficient efficacy to reduce worm numbers and associated disease burden is a promising management strategy to overcome these limitations. However, existing studies on the ES proteome of hookworms have mainly described proteins derived from the adult life stage which may preclude the opportunity to target larvae-specific parasitic processes. In this project, we use high resolution mass spectrometry to identify and compare ES proteins from the L3 stage as well as the adult stage of N. americanus and A. ceylanicum respectively.
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
