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ABSTRACT:
PROVIDER: PRJNA1226575 | ENA |
REPOSITORIES: ENA
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Project description:Analysis of microbial gene expression in response to physical and chemical gradients forming in the Columbia River, estuary, plume and coastal ocean was done in the context of the environmental data base. Gene expression was analyzed for 2,234 individual genes that were selected from fully sequenced genomes of 246 prokaryotic species (bacteria and archaea) as related to the nitrogen metabolism and carbon fixation. Seasonal molecular portraits of differential gene expression in prokaryotic communities during river-to-ocean transition were created using freshwater baseline samples (268, 270, 347, 002, 006, 207, 212). Total RNA was isolated from 64 filtered environmental water samples collected in the Columbia River coastal margin during 4 research cruises (14 from August, 2007; 17 from November, 2007; 18 from April, 2008; and 16 from June, 2008), and analyzed using microarray hybridization with the CombiMatrix 4X2K format. Microarray targets were prepared by reverse transcription of total RNA into fluorescently labeled cDNA. All samples were hybridized in duplicate, except samples 212 and 310 (hybridized in triplicate) and samples 336, 339, 50, 152, 157, and 199 (hybridized once). Sample location codes: number shows distance from the coast in km; CR, Columbia River transect in the plume and coastal ocean; NH, Newport Hydroline transect in the coastal ocean at Newport, Oregon; AST and HAM, Columbia River estuary locations near Astoria (river mile 7-9) and Hammond (river mile 5), respectively; TID, Columbia River estuary locations in the tidal basin (river mile 22-23); BA, river location at Beaver Army Dock (river mile 53) near Quincy, Oregon; UP, river location at mile 74.
2010-09-10 | E-GEOD-18303 | biostudies-arrayexpress
Project description:Analysis of microbial gene expression in response to physical and chemical gradients forming in the Columbia River, estuary, plume and coastal ocean was done in the context of the environmental data base. Gene expression was analyzed for 2,234 individual genes that were selected from fully sequenced genomes of 246 prokaryotic species (bacteria and archaea) as related to the nitrogen metabolism and carbon fixation. Seasonal molecular portraits of differential gene expression in prokaryotic communities during river-to-ocean transition were created using freshwater baseline samples (268, 270, 347, 002, 006, 207, 212).
2010-09-10 | GSE18303 | GEO
Project description:Amazon River plume small pigmented protist communities, May 2018 Raw sequence reads
| PRJNA701187 | ENA
Project description:Vestimentiferan tubeworms are representative inhabitants of deep-sea chemosynthetic ecosystems. The plume serves as the primary organ in these invertebrates without mouths and guts, facilitating direct metabolic exchange with their surrounding environments. In this study, we present a single-cell transcriptome atlas of Paraescarpia echinospica plume and this study is the initial single-cell transcriptome sequencing for the plume of the deep-sea tubeworm. We annotated six cell clusters including hemocytes, proliferative cells, muscle cells, epithelial cells, nerve1 cells, nerve2 cells and profiled genes involved in immunity and transport. This work will provide a foundation for further studies of tubeworm at the single-cell level.
2024-12-30 | GSE283331 | GEO
Project description:Vestimentiferan tubeworms are representative inhabitants of deep-sea chemosynthetic ecosystems. The plume serves as the primary organ in these invertebrates without mouths and guts, facilitating direct metabolic exchange with their surrounding environments. In this study, we present a single-cell transcriptome atlas of Paraescarpia echinospica plume and this study is the initial single-cell transcriptome sequencing for the plume of the deep-sea tubeworm. We annotated six cell clusters including hemocytes, proliferative cells, muscle cells, epithelial cells, nerve1 cells, nerve2 cells and profiled genes involved in immunity and transport. This work will provide a foundation for further studies of tubeworm at the single-cell level.
2024-12-30 | GSE281931 | GEO
Project description:Amazon river Myxozoa Oxford-Nanopore sequencing
| PRJNA1107121 | ENA