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DNA microarray analysis using liver from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

ABSTRACT: DNA microarray analysis using liver from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

PROVIDER: PRJNA1230077 | ENA |

REPOSITORIES: ENA

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DNA microarray analysis using duodenum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

Project description:DNA microarray analysis using duodenum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

| PRJNA1230066 | ENA

DNA microarray analysis using jejunum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

Project description:DNA microarray analysis using jejunum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

| PRJNA1230073 | ENA

DNA microarray analysis using liver from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

Project description:To understand the molecular mechanism by which IIAEK ameliorates hepatic and intestinal cholesterol metabolism, we performed DNA microarray analysis using liver, duodenal, and jejunal samples from the control and IIAEK groups of WT or IAP KO mice. We found that 1995 transcripts were identified in the livers of WT mice [WT, Control (WC) vs. WT, IIAEK (WI)] and 3802 in IAP-KO mice [IAP-KO, Control (KOC) vs. IAP-KO, IIAEK (KOI)] ( ≥1.2 fold-change, p < 0.05). Of these, 1729 transcripts fluctuated only in the WT group (WC vs. WI) and 3536 fluctuated only in the IAP-KO group (KOC vs. KOI).

2025-03-17 | GSE290764 | GEO

DNA microarray analysis using jejunum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

Project description:To understand the molecular mechanism by which IIAEK ameliorates hepatic and intestinal cholesterol metabolism, we performed DNA microarray analysis using liver, duodenal, and jejunal samples from the control and IIAEK groups of WT or IAP KO mice. We found that 3503 transcripts were identified in the jejunum of WT mice [WT, Control (WC) vs. WT, IIAEK (WI)] and 1388 in IAP-KO mice [IAP-KO, Control (KOC) vs. IAP-KO, IIAEK (KOI)] ( ≥1.2 fold-change, p < 0.05).

2025-03-17 | GSE290759 | GEO

DNA microarray analysis using duodenum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

Project description:To understand the molecular mechanism by which IIAEK ameliorates hepatic and intestinal cholesterol metabolism, we performed DNA microarray analysis using liver, duodenal, and jejunal samples from the control and IIAEK groups of WT or IAP KO mice. In total, 1406 transcripts were identified in the duodenum of WT mice ( ≥1.2 fold-change, p < 0.05). In contrast, we identified 777 transcripts in the duodenum of IAP-KO mice ( ≥1.2 fold-change, p < 0.05).

2025-03-17 | GSE290758 | GEO

In vivo protein-protein crosslinking in human lens through lys or protein N-terminus with Gln/Glu residues

Project description:In this study we identified protein-protein crosslinking through lys or protein N-terminus with Gln/Glu residues in aged human lens tissues. Such crosslinks could be reproduced experimentally by incubation of Glu- or Gln-containing peptides and their formation were consistent with an amino group attacking a glutarimide intermediate.

2023-08-01 | PXD022991 | Pride

Rice genes responsive to Glu

2016-04-01 | GSE78266 | GEO

Effects of physiological and synthetic IAP antagonism on c-IAP dependent signaling

Project description:Cellular inhibitor of apoptosis proteins 1 and 2 (c-IAP1/2) play central roles in signal transduction mediated by numerous receptors that participate in inflammatory and immune responses. In certain pathways, such as activation of NF-kB, their degradation is a major regulatory event and is physiologically induced by activation of receptors. Additionally, a number of synthetic compounds have been developed that also target the c-IAPs and induce their degradation. However, the extent of a synthetic IAP antagonist’s ability to mirror the transcriptional program by a physiological signal remains unclear. Here we take a systems approach to compare the transcriptional programs triggered by activation of CD30, a well-characterized receptor previously shown to induce the degradation of the c-IAPs, to SM-164, a synthetic IAP antagonist that specifically triggers c-IAP degradation. Employing a technique that allows the specific analysis of newly transcribed RNA, we have generated comparative transcriptome profiles for CD30 activation and SM-164 treatment. Analysis of these profiles revealed that the genes regulated by each stimulus were not completely shared, indicating novel functions of IAP antagonists and consequences of c-IAP1/2 degradation. The data identified a role for c-IAP1/2 in the regulation of the ribosome and protein synthesis, which was subsequently confirmed by biological assays. These findings expand our knowledge of the roles of c-IAP1/2 in signaling and provide insight into the mechanism of synthetic IAP antagonists, furthering our understanding of their therapeutic potential. This submission contains two different experiments. In experiment 1, cells were exposed to drug treament (DMSO or SM-164) for 3 hours. In experiment 2, cells were exposed to an adherent layer of CHO cells either expressing or not CD30L. Each treatment in each experiment was conducted once, leading to a total of 4 samples.

2015-02-09 | E-GEOD-64927 | biostudies-arrayexpress

Rice genes responsive to Glu

Project description:Treatment of rice roots with glutamate (Glu) induces systemic disease resistance against rice blast in leaves. To analyze the effect of Glu on the transcriptome of rice, rice roots were treated with Glu solution, and then fourth leaves were harvested and analyzed by Agilent rice microarray. Rice plants were treated with Glu (10 mM) or mock (water) solution and leaves were analyzed at 9 and 24 h in two biological replicates.

2016-04-01 | E-GEOD-78266 | biostudies-arrayexpress

Mammalian Pragmin regulates Src family kinases via the Glu-Pro-Ile-Tyr-Ala (EPIYA) motif that is exploited by bacterial effectors.

Project description:Several pathogenic bacteria have adopted effector proteins that, upon delivery into mammalian cells, undergo tyrosine phosphorylation at the Glu-Pro-Ile-Tyr-Ala (EPIYA) or EPIYA-like sequence motif by host kinases such as Src family kinases (SFKs). This EPIYA phosphorylation triggers complex formation of bacterial effectors with SH2 domain-containing proteins that results in perturbation of host cell signaling and subsequent pathogenesis. Although the presence of such an anomalous protein interaction suggests the existence of a mammalian EPIYA-containing protein whose function is mimicked or subverted by bacterial EPIYA effectors, no molecule that uses the EPIYA motif for biological function has so far been reported in mammals. Here we show that mammalian Pragmin/SgK223 undergoes tyrosine phosphorylation at the EPIYA motif by SFKs and thereby acquires the ability to interact with the SH2 domain of the C-terminal Src kinase (Csk), a negative regulator of SFKs. The Pragmin-Csk interaction prevents translocalization of Csk from the cytoplasm to the membrane and subsequent inactivation of membrane-associated SFKs. As a result, SFK activity is sustained in cells where Pragmin is phosphorylated at the EPIYA motif. Because EPIYA phosphorylation of Pragmin is mediated by SFKs, cytoplasmic sequestration of Csk by Pragmin establishes a positive feedback regulation of SFK activation. Remarkably, the Helicobacter pylori EPIYA effector CagA binds to the Csk SH2 domain in place of Pragmin and enforces membrane recruitment of Csk and subsequent inhibition of SFKs. This work identifies Pragmin as a mammalian EPIYA effector and suggests that bacterial EPIYA effectors target Pragmin to subvert SFKs for successful infection.

| S-EPMC3169139 | biostudies-literature

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