Project description:In this study we fed groups of female house flies one of two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) or control broth and used RNA-seq to determine differential gene expression at 4 hours post-feeding. Flies that ingested P. aeruginosa induced expression of genes encoding digestive proteins in the gut but did not induce AMPs. Flies in all treatment groups constitutively expressed some paralogs of lysozyme and AMP genes at the transcript level.
Project description:RNA-sequencing of the orbitofrontal cortex was performed in male and female rats following exposure to the limited bedding and nesting model of early life adversity.
Project description:To gain an understanding of the toxic effect of a commonly used solvent, flies were exposed to 0, 0.1, 0.2, or 0.4% v/v DMSO for 0, 2, 4, or 8 h. We performed compound exposure of 800 individual flies in 4 Whole Animal Feeding Flats (WAFFL), a novel 96 well system to house, feed, and harvest individual flies. This expression profiling was part of a set of the experiments performed to evaluate the suitability of the WAFFL for high throughput small compound screening in D. melanogaster. Treated flies and controls were used for poly A+ stranded mRNA library preparation and we performed high throughput RNA sequencing to determine the transcriptional changes due to DMSO treatment.
Project description:We treated mouse gastric organoids with Helicobacter pylori, Fusobacterium nucleatum, and Neisseria subflava, and extracted RNA after 4 hours. RNAs were applied to RNA sequencing analysis to investigate bacteria-specific effects on gastric epithelial cells.