Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Study on function and regulatory mechanism of a two-component signal transduction system cgtRS5 from Corynebacterium glutamicum

PROVIDER: PRJNA1246978 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR33001888_1.fastq.gz	Fastqsanger.gz
	SRR33001888_2.fastq.gz	Fastqsanger.gz
	SRR33001889_1.fastq.gz	Fastqsanger.gz
	SRR33001889_2.fastq.gz	Fastqsanger.gz
	SRR33001890_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Project description:Two-component signal transduction system-freeEscherichia coli

| PRJDB9625 | ENA

Corynebacterium glutamicum ATCC 13032

Project description:The Two-component Signal Transduction System CopRS of Corynebacterium glutamicum is Required for Adaptation to Copper-excess Stress

| PRJNA138577 | ENA

The Two-component Signal Transduction System CopRS of Corynebacterium glutamicum is Required for Adaptation to Copper-excess Stress

Project description:Copper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu2+ was studied using DNA microarrays and revealed 26 genes that showed a ≥3-fold increased mRNA level, including cg3280-cg3289. Several genes in this genomic region code for proteins presumably involved in the adaption to copper-induced stress, e. g. a multicopper oxidase and a copper-transport ATPase. In addition, this region includes the copRS genes (previously named cgtRS9) which encode a two-component signal transduction system composed of the histidine kinase CopS and the response regulator CopR. Deletion of the copRS genes increased the sensitivity of C. glutamicum towards copper ions, but not to other heavy metal ions. Using comparative transcriptome analysis of the ΔcopRS mutant and the wild type in combination with electrophoretic mobility shift assays and reporter gene studies the CopR regulon and the DNA-binding motif of CopR were identified. Evidence was obtained that CopR binds only to the intergenic region between cg3285 and cg3286 in the genome of C. glutamicum and activates expression of the divergently oriented gene clusters cg3285-cg3280 and cg3286-cg3289. Altogether, our data suggest that CopRS is the key regulatory system in C. glutamicum for the extracytoplasmic sensing of elevated copper ion concentrations and for induction of a set of genes capable of diminishing copper stress.

2011-09-05 | GSE27510 | GEO

Refutation: Structure and mechanism of the essential two-component signal-transduction system WalKR in Staphylococcus aureus.

Project description:Refutation: Structure and mechanism of the essential two-component signal-transduction system WalKR in Staphylococcus aureus.

| PRJEB14381 | ENA

Streptococcus pneumoniae

Project description:Investigation of pneumococcal two-component signal transduction system RR/HK06 in TIGR4

| PRJNA96343 | ENA

Streptococcus pneumoniae

Project description:Investigation of pneumococcal two-component signal transduction system RR/HK06 in D39

| PRJNA96331 | ENA

The Two-component Signal Transduction System CopRS of Corynebacterium glutamicum is Required for Adaptation to Copper-excess Stress

2011-09-04 | E-GEOD-27510 | biostudies-arrayexpress

Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

Project description:<Background> The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the P i starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ∆phoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR. <Results> DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to pstS promoter DNA in vitro. Gel mobility shift assays and mutational analysis of the pstS promoter region revealed that RamB binds to two sites localized at positions -74 to -88 and -9 to +2 with respect to the transcriptional start site of pstSCAB. Reporter gene studies supported the in vivo relevance of both binding sites for activation of pstSCAB by RamB. DNA microarray analysis revealed that expression of many Pi starvation inducible genes reached higher levels during the Pi starvation response on minimal medium with glucose as sole carbon source than in Pi starved acetate-grown C. glutamicum cells. <Conclusions> In C. glutamicum, RamB is involved in expression control of pstSCAB operon. Thus, transcriptional regulation of pstSCAB is complex involving activation by the phosphate-responsive two-component regulatory system PhoSR and the regulators of carbon metabolism GlxR and RamB.

2015-03-19 | GSE67012 | GEO

Corynebacterium glutamicum ATCC 13032

Project description:Control of heme homeostasis in Corynebacterium glutamicum by the two-component system HrrSA

| PRJNA135365 | ENA

Two-component signal transduction systems in Streptomyces and related organisms

Project description:Two-component signal transduction systems in Streptomyces and related organisms

| PRJNA110647 | ENA