Project description:To investigate the gene expression changes after Grx2 knockout, we isolated primary BMSCs from Grx2+/+ and Grx2-/- mice and passaged them to the 7th generation. Then, we performed gene expression analysis using RNA-seq data from six cell samples (three replicates per group).
Project description:To investigate the altered gene expression levels in MSR1 knockout and wild type macrophages in co-culture with BMSCs. Each group had a biological repeat (n=3). Results provide insight into the role of MSR1 in macrophages in a co-culture system.
Project description:A knockout clone has been generated for both FAM50A and FAM50B; knockout of the other gene is then performed and the transcriptome is analysed to look at the effect of dual gene loss.
Project description:The osteonecrotic area of steroid-induced avascular necrosis of the femoral head (SANFH) is a hypoxic microenvironment that leads to apoptosis of transplanted bone marrow mesenchymal stem cells (BMSCs). However, the underlying mechanism remains unclear. Here, we explore the mechanism of hypoxic-induced apoptosis of BMSCs, and use the mechanism to improve the transplantation efficacy of BMSCs. We analyzed LncRNA/mRNA expression profile of BMSCs under hypoxia conditions, and successfully screened the key long non-coding RNA AABR07053481 (LncAABR07053481) which mediated hypoxic apoptosis of BMSCs, we further determined its regulatory mechanism. Importantly, overexpression of LncAABR07053481 can improve the survival rate and repair efficacy of BMSCs under hypoxia conditions.
Project description:To investigate the altered gene expression levels in chondrocytes cocultured with BMSCs. Each group had a biological repeat (n=3). Results provide insight into the effect of chondrocytes cocultured with bone marrow stem cells
Project description:We determined the transcriptomic profile of bone-marrow derived macrophages (BMDMs) from LysMCre-mediated myeloid-specific Wdfy3 knockout mice and the Cre- littermate control mice.
Project description:LncRNA-NONMMUT002667 was idenified in BMSCs during aging. To identify the downstream molecules that mediate NONMMUT002667 induced BMSC location alteration, we FACS-sorted BMSCs from NONMMUT002667-KO mice and WT mice and performed a gene expression profile microarray.