Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Dental Pulp Stem Cell derived exosomes as effective biomaterials for anti-inflammation and regeneration in pulpitis

ABSTRACT: Human Dental Pulp Stem Cell derived exosomes as effective biomaterials for anti-inflammation and regeneration in pulpitis

PROVIDER: PRJNA1253670 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Human Dental Pulp Stem Cell derived exosomes as effective biomaterials for anti-inflammation and regeneration in pulpitis

Project description:The study investigates the effects of exosomes derived from human dental pulp stem cells (hDPC-Exos) on pulpitis, an inflammatory dental condition. The research demonstrates that hDPC-Exos exhibit significant anti-inflammatory and regenerative properties, making them effective biomaterials for treating pulpitis. Exosomes were shown to downregulate pro-inflammatory cytokines and chemokines, such as interleukin-1β and tumor necrosis factor-alpha, thus suppressing inflammation. Additionally, hDPC-Exos promoted angiogenesis and odontogenesis, facilitating recovery and regeneration of pulp tissue. These effects were observed through various analyses, including RNA sequencing and histological evaluations in an in vitro pulpitis model and a rat model of pulpitis. The findings suggest that hDPC-Exos hold potential as a cell-free therapeutic approach for oral inflammation and tissue regeneration.

2025-04-26 | GSE295186 | GEO

Human Dental Pulp Cell exosomes vs Human iPS Cell exosomes

Project description:Human Dental Pulp Cell exosomes vs Human iPS Cell exosomes

| PRJNA575247 | ENA

Human Dental Pulp Cell exosomes vs Human iPS Cell exosomes

Project description:Small vesicles, known as exosomes, are secreted from various cell types. Exosomes secreted by mesenchymal stem cells have therapeutic effects against a variety of diseases, and may be able to partially replace stem cell therapies. Previously, we established and characterized human leukocyte antigen (HLA) haplotype homo (HHH) dental pulp cell (DPC) lines from human wisdom teeth. In this report, we purified the exosomes secreted from HHH-DPCs and evaluated their therapeutic potential in a periodontitis model. The exosomes purified from HHH-DPCs showed homogeneous and spherical membrane structures, and showed low but significant expression of HLA class I molecules. The exosomes further promoted proliferation and migration in DPCs. A comparison of miRNAs revealed that the HHH-DPC exosomes contained higher levels of multiple Let-7 family miRNAs compared to HHH-induced pluripotent stem cell (iPSC)-derived exosomes. Finally, the HHH-DPC exosomes showed preventive effects in a mouse model of periodontitis induced by lipopolysaccharides (LPS). In summary, HHH-DPC exosomes expressed HLA molecules which may induce an immune response in HLA-mismatched transplantations. However, they successfully stimulated the proliferation and migration of cells and showed suppressive effects on LPS-induced periodontitis. Therefore, HHH-DPC exosomes show great potential for applications in periodontal treatments.

2022-04-01 | GSE138271 | GEO

Dental pulp microbiome

Project description:Dental pulp

| PRJEB50897 | ENA

Proteomics of exosomes derived from human dental pulp stem cells, human venous endothelial cells and human fibroblast

Project description:Proteomics of exosomes derived from human dental pulp stem cells, human venous endothelial cells and human fibroblasts

2024-11-04 | PXD057517 |

Gene expression analysis of human permanent dental pulp

Project description:Gene expression analysis data of human permanent dental pulp tissues

2016-11-22 | GSE75644 | GEO

Insulin-like Growth Factor Binding Protein 7 identified in aged dental pulp by single-cell RNA sequencing

Project description:Dental pulp plays a crucial role for dental health, and dental pulp aging influences their regenerative and reparative function. However, the underlying molecular mechanisms of dental pulp aging are not exhaustively understood, and thereby an in-depth and complete understanding of the aged dental pulp is of foremost importance. This study aimed to explore the heterogeneity of young and aged dental pulp tissue using single-cell RNA sequencing (scRNA-seq).

2024-12-15 | GSE274562 | GEO

Characterization of human dental pulp cells grown in chemically defined serum-free medium

Project description:Dental pulp cells obtained from several donors proliferated actively in a serum-free medium STK2. The growth rate of dental pulp cells from most donors was higher in the serum-free medium than that in a medium containing 10% serum. DNA microarray analyses showed that gene expression profile of dental pulp cells grown in the serum-free medium was similar to that of cells grown in a medium containing 10% serum. However, several genes related to cell proliferation were up-regulated in dental pulp cells grown in the serum-free medium.

2018-03-14 | GSE97199 | GEO

Induced neural cells from human dental pulp ameliorate functional recovery in a murine model of cerebral infarction

Project description:Human mesenchymal stem cells are a promising cell source for the treatment of stroke. Their primary mechanism of action occurs via neuroprotective effects by trophic factors, anti-inflammatory effects, and immunomodulation. However, the regeneration of damaged neuronal networks by cell transplantation remains still challenging. We hypothesized that cells induced to neural lineages would fit the niche, replace the lesion, and be more effective in improving symptoms compared with stem cells themselves. We investigated the characteristics of induced neural cells from human dental pulp tissue and compared the transplantation effects between these induced neural cells and uninduced dental pulp stem cells. Induced neural cells or dental pulp stem cells were intracerebrally transplanted 5 days after cerebral infarction induced by permanent middle cerebral artery occlusion in immunodeficient mice. Effects on functional recovery were also assessed through behavior testing. We used immunohistochemistry and neuron tracing to analyze the differentiation, axonal extension, and connectivity of transplanted cells to the host’s neural circuit. Transplantation of induced neural cells from human dental pulp ameliorated functional recovery after cerebral infarction compared with dental pulp stem cells. The induced neural cells comprised both neurons and glia and expressed functional voltage, and they were more related to neurogenesis in terms of transcriptomics. Induced neural cells had a higher viability than did dental pulp stem cells in hypoxic culture. We showed that induced neural cells from dental pulp tissue offer a novel therapeutic approach for recovery after cerebral infarction.

2021-09-01 | GSE174260 | GEO

SAHA Rat Dental Pulp

Project description:Transcriptional response of rat dental pulp cells (DPCs) cultured with SAHA at early and late mineralisation time points Transcript profiling of DPC identified several novel genes expression induced and supressed by HDACi at 24 hrs and 14 days under mineralising conditions. SAHA induces several members of the MMP family of endopepsidases (TIMP-1, MMP-9, MMP-13) and other members of the endochondral ossification pathway at 24 h. 8 experiemental parameters were analysed, each carried out in quadruplicate

2015-06-16 | E-GEOD-67175 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data