Project description:Baculovirus-encoded proteins can be efficiently produced following infection of different Drosophila cell lines. Epitope-tagged proteins produced in baculovirus-infected cells provide genome-wide profiles of the histone variant H2Av that are comparable to those produced by stably transformed cell lines. Keywords: Chromatin affinity-purification on microarray
Project description:Baculovirus-encoded proteins can be efficiently produced following infection of different Drosophila cell lines. Epitope-tagged proteins produced in baculovirus-infected cells provide genome-wide profiles of the histone variant H2Av that are comparable to those produced by stably transformed cell lines. Keywords: Chromatin affinity-purification on microarray All experiments were done using strepavidin or FLAG pulldown DNA cohybridized with total input DNA to the same array. Two channels per array, Cy5 and Cy3, were used in each experiment.
Project description:Identification of the interaction partners of the protein ecdysoneless (Ecd) in Drosophila melanogaster S2 cells as well as profiling of the changes in binding for mutant, truncated Ecd del34 protein.
