Xenopus laevis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA126571 | ENA |
REPOSITORIES: ENA
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Project description:Proteome analysis of blastocoel fluid recovered from Xenopus laevis embryo was carried out with highly sensitive nanoLC-MS/MS system using miniaturized spray columns.
2022-03-14 | PXD013096 | JPOST Repository
Project description:The experiment were perfomed as a part of our Vertebrate Evo-Devo project. The aim of the project is to compare transcription profiles of normal (unmanipulated, wild-type, whole embryo) vertebrate embryos. Total RNA was collected from wild type X.laevis whole embryos at 15 different stages (Stages:2, 5, 9, 11, 13, 17, 19, 21, 23, 26, 28, 31, 37/38, 43, 48), and hybridized to A-AFFY-163 Affymetrix Xenopus laevis Genome 2.0 Array. All the stages contains data from 2 biological replications. Each staged-samples consists of pooled total RNA from several whole embryos.
2011-08-01 | E-MTAB-369 | biostudies-arrayexpress
Project description:To adapt to its changing dietary environment, the digestive tract is extensively remodeled from the embryo to the adult during vertebrate development. Xenopus laevis metamorphosis is an excellent model system for studying mammalian gastrointestinal development and is used to determine the genes and signaling programs essential for intestinal development and maturation. The metamorphosing intestine can be divided into four distinct developmental time points and these were analyzed with X. laevis microarrays. Due to the high level of conservation in developmental signaling programs and homology to mammalian genes, annotations and bioinformatics analysis were based on human orthologs. Clustering of the expression patterns revealed co-expressed genes involved in essential cell processes such as apoptosis and proliferation. The two largest clusters of genes have expression peaks and troughs at the climax of metamorphosis respectively. Novel conserved gene ontology categories regulated during this period include transcriptional activity, signal transduction, and metabolic processes. Interestingly, the induced genes associated with metamorphic climax correlated with the gene expression peaks observed around birth in the mouse intestine. Thus both mouse and amphibian, share similarities at the molecular levels for intestinal maturation and remodeling, which appears to be under the influence of increasing levels of circulating thyroid hormone. Moreover, our genome-wide analysis of the intestine during development identified larval/embryo- and adult-specific genes. Detailed analysis revealed 17 larval specific genes that may represent molecular markers for human colonic cancers, while many adult specific genes are associated with dietary enzymes. This global developmental expression study provides the first detailed molecular description of intestinal remodeling and maturation during postembryonic development, which should help improve our understanding of intestinal organogenesis and human diseases. This study significantly contributes towards our understanding of the dynamics of molecular regulation during development and tissue renewal, which is important for future basic and clinical research and for medicinal applications. Time series experiment through natural metamorphosis of intestine in Xenopus laevis. Biological replicates: 3 replicates for each stage of major change during amphibian metamorphosis (except in the climax where 2 bioogical replicates were used). Universal reference design was used instead of dye-swap design for 2-color hybridizations.
2010-04-14 | E-GEOD-21303 | biostudies-arrayexpress
Project description:Affinity Purification Mass Spectrometry from Xenopus laevis animal caps. Anti-GFP mmunoprecipitation was performed from embryo explants expressing either GFP (control) or GFP-Lrrc56. MS-MS data were collected using a DDA top speed stepped HCD method with a 60 minute gradient. The lookup database was a Uniprot X.laevis reference proteome collapsed into EggNOG vertebrate-level orthology groups. Details of methods in accompanying manuscript.
2025-06-12 | MSV000098174 | MassIVE
Project description:Xenopus laevis laevis Transcriptome or Gene expression
| PRJNA247381 | ENA
Project description:We characterized the cilia proteome isolated from the multi-ciliated cells of Xenopus laevis embryo epidermis.
2019-11-15 | PXD010722 | Pride
Project description:Xenopus laevis Developing Eye Transcriptomes
| PRJNA1124041 | ENA