Project description:Gene expression profiling to address the effects of infection with Leishmania infantum during distinct clinical outcomes as active visceral leishmaniasis (VL), remission of disease and asymptomatic infection.
Project description:Leishmania infantum (Kinetoplastida:Trypanosomatidae) is the etiological agent of zoonotic visceral leishmaniasis in the Mediterranean basin. The motile promastigote stage infects the hematophagous sand fly vector host and amastigotes survives and multiplies within phagocytes of the mammalian host. Promastigotes are routinely cultured in liquid undefined media and are considered to mimic the environment within the sand fly gut. We have put this to the test by high-throughput gene expression profiling by shotgun DNA microarrays generated in our laboratory. This has been possible thanks to RNA amplification.
Project description:Gene expression profiling to address the effects of infection with Leishmania infantum during distinct clinical outcomes as active visceral leishmaniasis (VL), remission of disease and asymptomatic infection. Total RNA was extracted from whole-blood lysates obtained from VL patients, treated VL patients, asymptomatic individuals and uninfected controls. The aim was to evaluate gene expression signatures associated with protective and pathological responses.
Project description:This was an in vitro experimental study of monocytes-derived macrophages from blood samples of BSCL individuals (type I - AGPAT2, type II - BSCL2, and heterozygous - BSCL2), and healthy controls (WT). Samples were infected with L. infantum. In the control group, there are monocytes-derived macrophages from individuals WT with and without a history of visceral leishmaniasis (VL). RNA-seq was done to identify the different group responses to the infection.
Project description:Leishmania infantum is the causative agent of visceral leishmaniasis in Latin America, a lethal disease if misdiagnosed or left untreated. The ubiquitin proteasome system (UPS) is the main regulator of intracellular proteolysis in eukaryotes and is required for parasite's to life cycle and infection. E3 ubiquitin ligases play important role in UPS and Cullin-1-RING ligases (CRL1) are the largest and most researched family of E3 in eukaryotes. CRL1 complex is made up of SKP1, Cullin1, RBX1, and F-box proteins. This complex is poorly studied in Leishmania. We investigated the interactome of CRL1 complex in L. infantum combining in vitro and in cellulo experiments to identify the interactome of Cullin1 and SKP1 through affinity purification followed by mass spectrometry analysis.
Project description:Leishmania (L.) infantum is the etiologic agent of visceral leishmaniasis (VL). In Brazil represents a serious public health problem. Studies have shown that regulation of immune response appears to depend on miRNAs. In canine VL due to cell immune suppression being determinant of disease progression, knowledge of miRNAs may be important for the pattern of change in immune response. Here, we suggest that post-transcriptional regulation, mediated by miRNAs, may play a role in immune response of dogs with VL.
