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Mus musculus

ABSTRACT: Expression data from mouse colon tissue response to DSS induction at day 0, 2, 4 and 6

OTHER RELATED OMICS DATASETS IN: E-GEOD-22307

PROVIDER: PRJNA127385 | ENA |

REPOSITORIES: ENA

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E-GEOD-22307

Project description:Transcription profiling by array of mouse colon tissues under dextran sodium sulphate induction for 2, 4 and 6 days against uninduced controls at day 0

2015-01-06 | E-GEOD-22307 | ExpressionAtlas

E-GEOD-83550

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A functional genomics predictive network model identifies regulators of inflammatory bowel disease: Ribo-zero RNAseq mouse distal colon DSS Colitis model of genetically engineered mice

Project description:Ribo-zero RNAseq experiments were carried out with mouse distal colon DSS Colitis model of genetically engineered mice. For each mouse line for: Gpsm3 (3% DSS), Aif1 (3% DSS), Nckap1l (Hem1), Dock2 (3% DSS), Dok3 (3.5% DSS) DSS treatment for five days followed by water for 5 days, (7 days for Nckap1l). Tissue was collected at Day 10 (Day 12 for Nckap1l (HEM1)).

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Expression data from mouse colon tissue response to DSS induction at day 0, 2, 4 and 6

Project description:Temporal genome profiling of DSS colitis The DSS induced mouse colitis model is often used to emulate Ulcerative Colitis (UC) in order understand pathophysiological mechanism of inflammatory bowel disease (IBD). Given the progressive nature of IBD, colon tissue gene expression changes during the evolution of disease, and knowing the changes in gene expression profiles could indentify potential diagnostic markers or additional therapeutic targets for colitis. Therefore, we performed temporal genome expression profiling analysis using the Affymetrix genome wide microarray system to identify broad scale changes in gene expression associated with the development of colitis. Keywords: Expression time course of mouse colon tissue induced by 3% DSS. C57BL/6J mice were given 3% DSS in the drinking water and tissues from individual cohorts were collected at days 0, 2, 4 and 6. Total RNA were extracted from the colon tissue and detected by Affymerix GeneChip Mouse Genome 430 2.0 Array.

2010-10-13 | E-GEOD-22307 | biostudies-arrayexpress

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10X-single cell seq of B cells during colitis (d0 and d14)

Project description:B cells expand during the recovery after DSS-induced colonic inflammation. To investigate if a specific subtype is expanding and to analyse the transcriptional profile of these cells a transcriptional analysis on single cell level was carried out. In detail, B cells were isolated from the colon of mice on day 0 (no treatment) or day 14 (recovery) after 7 days of DSS treatment by flow cytometric sorting and analyzed by 10X sequencing.

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Mus musculus

Project description:Expression data from mouse colon tissue response to T cell transfer at week 0, 2, 4 and 6

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Expression data from untreated and DSS-treated Adamts12 WT and KO mice

Project description:Adamts12-deficient mice undergo more severe colitis than WT mice after induction with DSS. We used microarrays to determine the gene expression differences between Adamts12-deficient and WT mice during ulcerative colitis induced with DSS (dextran sodium sulfate) Fragments of distal colon from DSS-treated (2% DSS during 7 days and 1 day of recovery) and untreated Adamts12-deficient and WT mice were obtained for RNA extraction and hybridiztion with Affymetrix microarrays

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