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Thermus thermophilus HB8

ABSTRACT: mRNA expression in wild type and TTHA0175-deficient Thermus thermophilus HB8 strains rich medium with growth temperature shift from 70C to 45C

OTHER RELATED OMICS DATASETS IN: GSE21875

PROVIDER: PRJNA129465 | ENA |

REPOSITORIES: ENA

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Expression profile of crp-deficient Thermus thermophilus HB8 strain

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mRNA expression in ttcsp1 deletion mutant and wild type Thermus thermophilus HB8 strains grown on a rich medium

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TTHB212 (anti-sigmaE)-deficient Thermus thermophilus HB8 strain: time course

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70°C. Keywords: time course

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course

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mRNA expression in wild type and TTHA0175-deficient Thermus thermophilus HB8 strains; rich medium with growth temperature shift from 70C to 45C

Project description:In order to find function of TTHA0175 protein, we analyzed altered expression profile of the total mRNA of TTHA0175-deficient Thermus thermophilus HB8 strain grown in a rich (TT) medium after shifted down the growth temperature from 70 oC to 45 oC, and compared to that in the cases of wild type.

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Transcription profiling of Thermus thermophilus HB8 wild-type strain grown on a rich medium

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Experiment Overall Design: wild-type T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0min and 30min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression in thewild-type cell between 45oC and 70oC, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2009-01-09 | E-GEOD-12437 | biostudies-arrayexpress

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