Project description:Interactome of OCIAD1-FLAG was studied. TMT-based relative quantification of protein levels was performed in eluates from mitochondria of HEK293 cells that expressed OCIAD1-FLAG vs empty vector and from mitochondria of OCIAD1-KO cells that expressed OCIAD1-FLAG vs empty vector.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression. Two-condition experiment, Normoxic MSCs vs. Hypoxic MSCs.