Project description:Treatment with MicroRNA cluster B and C increase the iPS efficiency We used microarrays to identify changes induced by MicroRNA clusters in the iPS process
Project description:Treatment with MicroRNA cluster B and C increase the iPS efficiency We used microarrays to identify changes induced by MicroRNA clusters in the iPS process One group were MEFs infected with SKO factors, plus MicroRNA cluster B, C or control blank virus. The other group were MEFs infected with MicroRNA cluster B, C or control blank virus only.TRIZOL cell lysates were prepared at D4 and D8.
Project description:In this study we focus on the identification of new genes tentatively involved in sporulation and those that influence properties of spores and their ability to germinate. To this end, the sporulation stages of C. perfringens enterotoxic strain SM101 were characterized based on morphological characteristics and biological indicators. Subsequently, whole genome expression profiling during key stages of the sporulation process was performed using DNA microarrays, and genes were clustered based on their time-course expression profiles during sporulation. The majority of previously characterized C. perfringens germination genes showed upregulated expression profiles in time during sporulation and mainly belonged to two clusters of genes.
