Project description:This SuperSeries is composed of the following subset Series: GSE24692: chip in silencing suppressor plants or in mirna plants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing GSE24693: gene profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing GSE26739: Expression profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing Refer to individual Series
Project description:chip in silencing suppressor plants or in mirna plants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing
Project description:Expression profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing
Project description:gene profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing
Project description:au07-12_geminiselsup - gemsup - Determine the level of expresison in transgenic plants expressing geminviral gene silencing suppressors. - Transgenic lines of Arabidopsis overexpressing three different geminiviral silencing suppressors: C2, C4 and V2 were grown in adition to a control and their total RNA was extracted in order to study their genetic expressions profiles. Keywords: gene knock in (transgenic)
Project description:au09-04_geminisilsup2 - gemsupv2 - Determine the level of expression in transgenic plants expressing geminviral gene silencing suppressors. One transgenic line of Arabidopsis overexpressing the geminiviral silencing suppressor V2 was grown in addition to a control, and their total RNA was extracted in order to study their genetic expression profiles.
Project description:Parallel RNA silencing pathways regulate gene expression in plants, either by transcriptional gene silencing via RNA-dependent DNA methylation (RdDM), or by post-transcriptional silencing targeting mRNAs. Both pathways rely on distinct Dicer-like proteins to cleave double-stranded RNA into small-interfering RNAs. Experiments to determine the subcellular localization of Dicer-like proteins in Arabidopsis revealed that DCL4 is predominantly expressed as a transcriptional start site isoform that encodes a cytoplasmic protein. A second, longer DCL4 transcript isoform encodes a nuclear-localization signal and its expression is repressed by DNA methylation. Consequently this isoform is induced when promoter methylation decreases due to infection with a bacterial pathogen or during silique development. Nuclear DCL4 produces unique populations of small RNAs, called DCL4NLS isoform-dependent siRNAs (disiRNAs), which function via a post-transcriptional silencing effector, but whose precursors are generated by the RdDM pathway. Arabidopsis cells can thus respond to genome methylation changes by modulating DCL4 localization, which in turn recruits PTGS factors to reinforce RNA silencing.
Project description:au07-12_geminiselsup - gemsup - Determine the level of expresison in transgenic plants expressing geminviral gene silencing suppressors. - Transgenic lines of Arabidopsis overexpressing three different geminiviral silencing suppressors: C2, C4 and V2 were grown in adition to a control and their total RNA was extracted in order to study their genetic expressions profiles. Keywords: gene knock in (transgenic) 9 dye-swap - CATMA arrays
Project description:au10-09_gemsupv2ts-7 - gemsupv2ts-7 - Determine the level of expression in transgenic plants expressing geminiviral gene silencing suppressors. - One transgenic line of Arabidopsis overexpressing the geminiviral silencing suppressor V2TS was grown in adition to a control and their total RNA was extracted in order to study its genetic expression profile.
Project description:au09-04_geminisilsup2 - gemsupv2 - Determine the level of expression in transgenic plants expressing geminviral gene silencing suppressors. One transgenic line of Arabidopsis overexpressing the geminiviral silencing suppressor V2 was grown in addition to a control, and their total RNA was extracted in order to study their genetic expression profiles. 3 dye-swaps - gene knock-in (transgenic).
