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PROVIDER: PRJNA132195 | ENA |
REPOSITORIES: ENA
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Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. Differential genes expression by both Sohlh1 and Sohlh2 deficiency in mouse newborn ovaries was accessed using microarray. RNA samples from Sohlh1/ Sohlh2 double knockout and wild-type newborn ovaries were arrayed on the Illumina beadchip mouse WG-6 2.0. Total RNA isolated from wildtype and Sohlh1/Sohlh2 double KO mouse newborn ovary were used to run Illumina BeadChip MouseWG-6 2.0 arrays.
2010-10-22 | E-GEOD-24815 | biostudies-arrayexpress
Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. Differential genes expression by both Sohlh1 and Sohlh2 deficiency in mouse newborn ovaries was accessed using microarray. RNA samples from Sohlh1/ Sohlh2 double knockout and wild-type newborn ovaries were arrayed on the Illumina beadchip mouse WG-6 2.0.
2010-10-22 | GSE24815 | GEO
Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. We discovered that Sohlh1 and Sohlh2 knockout females lose oocytes after birth and few remains by postnatal day 14. Here, we show that many genes preferentially expressed in the oocytes are misregulated by Sohlh1 and/or Sohlh2 deficiency. Total RNA samples isolated from wildtype, Sohlh1 KO and Sohlh2 KO mouse newborn ovaries were arrayed on the Illumina BeadChip MouseWG-6 2.0 arrays. We have 4 replicates for wildtype and Sohlh1 KO and 3 replicates for Sohlh2 KO.
2010-10-22 | E-GEOD-24816 | biostudies-arrayexpress
Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. We discovered that Sohlh1 and Sohlh2 knockout females lose oocytes after birth and few remains by postnatal day 14. Here, we show that many genes preferentially expressed in the oocytes are misregulated by Sohlh1 and/or Sohlh2 deficiency.
2010-10-22 | GSE24816 | GEO
Project description:Germ-cell transcription factors control gene networks that regulate primordial follicle formation and oocyte differentiation during early, postnatal mouse oogenesis. Taking advantage of gene-edited mice lacking transcription factors expressed in female germ cells, we analyzed global gene expression profiles in perinatal ovaries from wildtype, FiglaNull, Lhx8Null and SohlhNull mice. Figla deficiency dysregulates expression of meiosis-related genes (e.g., Sycp3, Rad51 and Msy2) and a variety of genes (e.g., Nobox, Lhx8, Taf4b, Sohlh1, Sohlh2 and Gdf9) associated with oocyte growth and differentiation. The absence of FIGLA significantly impedes meiotic progression, causes DNA damage and results in oocyte apoptosis. Moreover, we find that FIGLA and other transcriptional regulators (e.g., NOBOX, LHX8, SOHLH1 and SOHLH2) are co-expressed in the same subset of germ cells in perinatal ovaries and Figla ablation dramatically disrupts KIT, NOBOX, LHX8, SOHLH1 and SOHLH2 expression. In addition, not only do FIGLA, SOHLH1 and LHX8 cross-regulate each other, they also cooperate by direct interaction with each during early oocyte development and share downstream gene targets. Thus, our findings substantiate a major role for FIGLA, LHX8 and SOHLH1 as multifunctional regulators of networks necessary for oocyte maintenance and differentiation during early folliculogenesis.
2019-11-06 | GSE139966 | GEO
Project description:Ovarian folliculogenesis in mammals is a complex process involving cross talk between germ and somatic cells. Carefully orchestrated expression of transcription factors, cell adhesion molecules and growth factors are required for success. We have identified a germ-cell specific basic helix-loop-helix transcription factor, FIGLA (Factor In GermLine, Alpha) and demonstrated its involvement in two independent developmental processes: formation of primordial follicle and coordinate expression of zona pellucida genes. Taking advantage of Figla null mouse line, we have used microarrays to identify potential downstream target genes. Using high stringent cut offs, we find that FIGLA functions a key regulatory molecule in coordinating expression of the NALP family of genes, genes of known oocyte-specific expression and a set of functionally un-annotated genes. These data implicate FIGLA as a central regulator of oocyte-specific genes that play role in folliculogenesis and early development. We analyzed 8 groups of arrays, 26 arrays total. Four arrays of WT newborn ovary RNA (cy5) versus KO newborn ovary RNA (Cy3), then four arrays of the same samples dye-reversed. Three arrays of WT E17.5 ovary RNA (cy5) versus KO E17.5 ovary RNA (Cy3), then three arrays of the same samples dye-reversed. Three arrays of WT E14.5 ovary RNA (cy5) versus KO E14.5 ovary RNA (Cy3), then three arrays of the same samples dye-reversed. Three arrays of WT E12.5 ovary RNA (cy5) versus KO E12.5 ovary RNA (Cy3), then three arrays of the same samples dye-reversed.
2007-08-17 | E-GEOD-5558 | biostudies-arrayexpress
Project description:ERβ is predominantly expressed in granulosa cells of the ovary, where its important for normal ovarian function and plays a vital role in folliculogenesis. However, the genome wide binding landscape of ERβ in the ovary was not determined and its interaction targets are unknown. To characterize the mechanism of ERβ, we performed ChIP-Seq using endogenous ERβ expression and mapped the cistrome of ERβ in the ovary using a validated antibody.
2023-11-17 | GSE203391 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE24815: Transcriptional changes in Sohlh1/Sohlh2 double knockout mouse newborn ovaries GSE24816: Transcriptional changes in Sohlh1 knockout and Sohlh2 knockout mouse newborn ovaries Refer to individual Series
2010-10-22 | E-GEOD-24817 | biostudies-arrayexpress
Project description:Background Correct achievement of early ovarian folliculogenesis is a crucial phase for further ovarian function. This process is closely regulated by cell-cell interactions and coordinated expression of genes from oocyte and granulosa cells. But, despite of the large number of studies, little is known about the precise gene expression patterns driving early folliculogenesis. The experimental limitations concerned the very small size of these follicles and the mixture of the different developmental stages within an ovary that make the study of isolated follicular components much more difficult. The recently developed laser capture microdissection (LCM) technique coupled with microarrays experiments is promising in addressing the molecular specificity of each follicular compartment. Nevertheless, the isolation of unique cells or group of cells is still challenging to maintain RNA quality during this process and to obtain sufficient amount of RNA. In this study, we described a method allowing the analysis of oocyte and granulosa cells gene expression during the first stages of sheep early folliculogenesis. Results First we developed a new fixation protocol using a frizzed 70% ethanol fixation solution that ensures correct single cell capture and RNA integrity during microdissection time. After LCM capture of the compartments and follicular stages, RNA extraction and amplification, the expression of 6 oocyte-specific genes (SOHLH2, MAEL, MATER, VASA, GDF9, BMP15) and 3 granulosa cell-specific genes (KITLG, GATA4, AMH) confirmed the purity of the samples and documented their ovine expression profiles. Then, using bovine Affymetrix chip, we identified for the first time, a global gene expression for each follicular compartment during early developmental stages. Particularly the granulosa cell data set is quite unique. 1050 granulosa cell specific transcripts compared to oocyte and 759 oocyte specific transcripts were detected. The analysis of the expression of 2 genes (SIRT7, FST) confirmed this specificity of expression. Finally, the integration of the data stated the 3 main physiological events involved in early folliculogenesis and provided descriptive elements that confirmed the relevance and the potential of the LCM-derived RNAs. Conclusions This method should contribute through an additional genome wide expression profiling to give insights on molecular mechanisms involved in stage transitions and cell type interplays. The 2 ovine follicular compartments (i.e. granulosa cells (G) and oocytes (O) were captured using LCM technology for each early stage (primordial (Pd), primary (Pm), secondary (Sec) follicles. The RNA of each group was extracted using Picopure RNA Isolation kit (Arcturus) and subjected to 2 round T7 amplification (RiboAmp®HS PLUS kit, Arcturus). Ovine microarray experiments were performed using the Affymetrix Bovine Expression Array. First the quality of the cross-species hybridizations was checked by comparison of hybridization data of ovine fetal ovary RNA with bovine fetal ovary ones, generated with the Affymetrix standard protocol (protocole 1). Then, three biotin-labeling protocols were compared from ovine fetal ovary total RNA: protocol 1; protocol 2 (Biotin-labeled cRNAs were synthesized following Affymetrix protocol using the second-round cDNAs from RiboAmp®HS kit as templates); protocol 3 (Arcturus biotin turboTM labeling kit from aRNA after the 2 round amplification using RiboAmp®HS kit). Last , one LCM-derived aRNA sample of each group was labeled using the Arcturus biotin turboTM labeling kit (protocol 3) and hybridized to Affymetrix Bovine Expression arrays. Images were interpreted using Microarray Suite version 5.0 (MAS 5.0) in GCOS with scaling (100) and without normalization.
2011-07-01 | E-GEOD-25652 | biostudies-arrayexpress
Project description:Y box-binding protein 1 (YB-1; Ybx1/ybx1) regulates transcription and translation of targeted genes by DNA/RNA-binding. Our previous proteomic study demonstrated a dramatic drop in Ybx1 protein during follicle activation in zebrafish ovary. In this study, we created an ybx1 mutant with CRISPR/Cas9, and showed that the folliculogenesis in the mutant ovary (ybx1-/-) was blocked at pre-vitellogenic (PV)-to-early vitellogenic (EV) transition, leading to small ovaries. The mutant females were therefore sub-fertile with reduced fecundity. RNA-seq and Western blot analyses identified a variety of genes that showed differential expression between mutant ovary (ybx1-/-) and the control (ybx1+/-) including cdkn1a (p21). Disruption of cdkn1a resulted in embryonic lethality. In p21 heterozygotes (cdkn1a+/-), however, follicle activation and maturation in the ovary were both enhanced in contrast to ybx1 mutant (ybx1-/-). Interestingly, partial loss of p21 in heterozygotes (cdkn1a+/-) could rescue the phenotype of ybx1 mutant (ybx1-/-). Folliculogenesis resumed in ybx1-/-;p21+/- females with normal follicle activation, in contrast to the PV-EV blockade in ybx1-/- mutant. Interestingly, the follicle cells from the ybx1-/- mutant follicles displayed a poor proliferative activity in vitro; however, the cells from the ybx1-/-p21+/- follicles resumed normal proliferation compared to that from the wildtype fish. In summary, we demonstrated in this study that Ybx1 played a gatekeeping role in controlling PV-to-EV transition and it might act by suppressing the expression of cdkn1a, a cell cycle inhibitor.
2024-10-27 | GSE239623 | GEO