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Saccharomyces cerevisiae YJM789

ABSTRACT: WGS sequencing and assembly

PROVIDER: PRJNA13304 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	AAFW02.dat.gz	Other
	AAFW02.fasta.gz	Fasta.gz
	AAFW02.master.dat	Other
	AAFW02.dat.gz	Other

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Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789.

Project description:We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensive phenotypic differences to the laboratory reference strain, including growth at high temperature and deadly virulence in mouse models. Here we show that the approximately 12-Mb genome of YJM789 contains approximately 60,000 SNPs and approximately 6,000 indels with respect to the reference S288c genome, leading to protein polymorphisms with a few known cases of phenotypic changes. Several ORFs are found to be unique to YJM789, some of which might have been acquired through horizontal transfer. Localized regions of high polymorphism density are scattered over the genome, in some cases spanning multiple ORFs and in others concentrated within single genes. The sequence of YJM789 contains clues to pathogenicity and spurs the development of more powerful approaches to dissecting the genetic basis of complex hereditary traits.

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