Project description:We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensive phenotypic differences to the laboratory reference strain, including growth at high temperature and deadly virulence in mouse models. Here we show that the approximately 12-Mb genome of YJM789 contains approximately 60,000 SNPs and approximately 6,000 indels with respect to the reference S288c genome, leading to protein polymorphisms with a few known cases of phenotypic changes. Several ORFs are found to be unique to YJM789, some of which might have been acquired through horizontal transfer. Localized regions of high polymorphism density are scattered over the genome, in some cases spanning multiple ORFs and in others concentrated within single genes. The sequence of YJM789 contains clues to pathogenicity and spurs the development of more powerful approaches to dissecting the genetic basis of complex hereditary traits.

| S-EPMC1933262 | biostudies-literature

DEtail-seq profiled meiotic DSBs in Saccharomyces cerevisiae

Project description:SPO11-promoted DNA double-strand breaks (DSBs) formation is a crucial step for meiotic recombination, and it is indispensable to detect the broken DNA ends accurately for dissecting the molecular mechanisms behind. Here, we report a novel technique, named DEtail-seq (DNA End tailing followed by sequencing), that can directly and quantitatively capture the meiotic DSB 3’ overhang hotspots at single-nucleotide resolution.

2023-01-08 | GSE154226 | GEO

Saccharomyces cerevisiae

Project description:Spo11-accessory proteins link DNA double-strand break sites to the chromosome axis in early meiotic recombination

| PRJNA140909 | ENA

Saccharomyces cerevisiae YJM789

Project description:WGS sequencing and assembly

| PRJNA13304 | ENA

Saccharomyces cerevisiae YJM789

Project description:The sequencing of Saccharomyces cerevisiae strains.

| PRJNA215584 | ENA

Saccharomyces cerevisiae S288c x YJM789

Project description:Down-regulation of Rad51 Activity During Meiosis in Yeast Prevents Competition with Dmc1 for Repair of Double-Strand Breaks