Project description:To quantify gene expression differences in olfactory epithelium between the mouse (Mus musculus) and the Nile rat (Arvicanthis niloticus), paired-end RNA sequencing (RNA-seq) was used to profile olfactory epithelium transcriptomes of six Nile rats and six mice (C57BL/6J) (one male and one female at the age of 8, 12, and 16 weeks for each species).
Project description:Mice lacking the transcription factor Fezf1 exhibit defects in the structural and molecular organiztion of their olfactory system. To invetigate this at the level of gene expression, we isolated Fezf1 expressing cells by FACS from the MOE of Fezf1+/- or Fezf1-/- animals and compared their gene expression profiles.
Project description:Mice lacking the transcription factor Fezf1 exhibit defects in the structural and molecular organiztion of their olfactory system. To invetigate this at the level of gene expression, we isolated Fezf1 expressing cells by FACS from the MOE of Fezf1+/- or Fezf1-/- animals and compared their gene expression profiles. The targeted Fezf1 allele contains an EGFP coding sequence under control of the endogenous Fezf1 promoter. We utilized this to isolate Fezf1+/- or Fezf1 -/- cells from the microdissected MOE tissue of E18.5 mouse embryos. Total RNA was isolated from these cells and gene expression examined by Affymetrix 1.0 ST array.
Project description:This SuperSeries is composed of the following subset Series: GSE39514: Effects of H2be ectopic over-expression on gene expression in the main olfactory epithelium (MOE) of 5-week old mice. GSE39515: Effects of H2be loss of function on gene expression in the main olfactory epithelium (MOE) of 6-month old mice. GSE39516: Effects of H2be loss of function on gene expression changes in the main olfactory epithelium (MOE) as a result of activity deprivation through unilateral naris occlusion (UNO). GSE39517: Genome-wide location analysis of FLAG-H2BE Refer to individual Series
