Project description:The C5/TRAF1 locus is a well validated genetic risk factor for RA. Upon further investigation of this region we recently discovered that the intergenic region between these two immune-related genes is activelly transcribed in a variety of tissues and is up-regulated in PBMCs, monocytes, B- and T-cells upon immune stimulation. The present study aimed to characterize the biological function of this newly identified C5/TRAF1 intergenic transcript, by analysing the effect of its knockdow on the gene expression profiles of Huh7 and THP1 cells.
Project description:RNA-seq dataset of TRAFD1 knockdown in THP-1 cell lines compared to THP1 cell lines treated with non targeting siRNA (SCR) and untreated cells (WT), under unstimulated and stimulated (LPS) conditions.
Project description:Reduction of NEAT1 transcript associated with Cell type-specific differential expression. The Knockdown of NEAT1 in Huh7 cells modulated antiviral response and dengue virus replication via the RIG-I pathway. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.
Project description:We report the impact of altered CMPK2 expression in THP1 macrophages. CMPK2 silencing THP1 macrophages (CM) were constructed by stably expressing siRNA against CMPK2. Scrambled siRNA expressing THP1 cells were used as control (SC). THP1 macrophages stably expressing CMPK2 fused with mCherry was used as CMPK2 overexpressing macrophages (OE). Control cells were constructed by overexpressing mCherry alone (VC). The RNA sequencing was used to identify genes that are differentially expressed in CMPK2 modified THP1 macrophages.
Project description:To determine the effects of UBL5 on genome-wide gene expression, we used UBL5 siRNA to knock down UBL5 in HepG2 and Huh7 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq.
