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Schizosaccharomyces japonicus

ABSTRACT: Genome used in a comparative analysis to elucidate fission yeast evolution

PROVIDER: PRJNA13637 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	DRR003305_1.fastq.gz	Fastqsanger.gz
	DRR003305_2.fastq.gz	Fastqsanger.gz
	DRR003306_1.fastq.gz	Fastqsanger.gz
	DRR003306_2.fastq.gz	Fastqsanger.gz

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small RNA discovery in fission yeast Schizosaccharomyces japonicus

Project description:We used massively parallel sequencing to discover and characterize small RNAs (sRNAs) from fission yeast Schizosaccharomyces japonicus. We found that, unlike in related S. pombe, a substantial fraction of sRNAs maps to transposons, both telomeric and centromeric.

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RNA sequencing of Schizosaccharomyces pombe cells with the replaced fatty acid synthase complex from Schizosaccharomyces japonicus

Project description:We have identified that these two organisms have quite distinct phospholipid structures - S. pombe phospholipids display canonical symmetric long acyl chains (18 and/ or 16 carbon atoms), and in S. japonicus significant fraction of phospholipids are asymmetrical - one acyl chain is 18 carbon atoms and second one is short - only 10 carbon atoms. Medium chain fatty acid like C10:0 are critical for S. japonicus physiology and are synthesised by the cytosolic fatty acid synthase (FAS) complex. Such findings lead us to address the functional role of these asymmetric lipids and we exchanged FAS between the two fission yeast species. We observed that in S. pombe expressing solely japonicus FAS, the membrane is functionally impaired in a number of ways (e.g. endocytosis, mitosis etc etc) and moreover, it seems that at lower temperatures (such as 24C) the phenotype is more severe compared to the higher temperatures like 30 and 36. We believe that comparative transcriptomic analysis will shed light on what is going on in S. pombe cells with exchanged FAS and therefore displaying an altered lipidome.

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Genome wide map of H3K9me2 in fission yeast Schizosaccharomyces pombe.

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Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe

Project description:Genome wide map of heterochromatin state in fission yeast Schizosaccharomyces pombe via 4 different strains

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Heat-shock molecular chaperone Hsp90 is involved in heterochromatin assembly in Schizosaccharomyces pombe.

Project description:Here, we report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. We generated genome-wide H3K9me2 maps of fission yeast mutants in swo1-26 (temperature sensitive, ts) cells at 25℃ and 37℃. We find that H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects.

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