MiRNA expression in NK cells
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ABSTRACT:
PROVIDER: PRJNA137199 | ENA |
REPOSITORIES: ENA
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Project description:The goal of this study is to characterize the miRNA expression in both freshly isolated and cultured NK cells from both mice and humans. (2 samples from mouse cells (naive and cultured), 2 from human cells (naïve and cell line), 4 samples total)
2011-02-23 | GSE27249 | GEO
Project description:The goal of this study is to characterize the miRNA expression in both freshly isolated and cultured NK cells from both mice and humans. (2 samples from mouse cells (naive and cultured), 2 from human cells (naïve and cell line), 4 samples total) At this point, this experiment involves one replicate of each of the samples using dual channels to analyze freshly isolated cells and cultured cells on the same custom printed slide. (intended only to gain preliminary insight into miRNA expression in NK cells for further analysis) SUPPLEMENTARY FILE: File includes normalized total intensity and log2 ratio (representing RNA from freshly isolated cells-Cy5/cultured cells-Cy3[mouse] or freshly isolated cells-Cy5/cell line-Cy3[human]).
2011-02-23 | E-GEOD-27249 | biostudies-arrayexpress
Project description:Natural Killer (NK) cells present natural cytotoxicity against tumor cells, although their activity is increased after activation.NK cell activation depends on a complex intracellular signaling process mediated by activating and inhibitory receptors and the functional outcome depends on the integration of the activating and inhibitory signals received. Soluble cytokines and/or ligands on target cells bind the NK cell receptors, and hence, influence the final NK cell response: attack versus ignorance. We used microarrays to detail the global programme of gene expression underlying NK cell activation by IL-2, a MHC-I-deficient target cell (K562)+IL-2 and an EBV-target cell (R69). PBLs from 4 different donors were activated by 100 U/ml IL-2; K562+IL-2 or R69 cells. After 5 days we obtained RNA and miRNA from naïve NK cells or from NK cells activated with the above mentioned stimuli, with more than 90% of purity. The 16 RNA samples were used to generate cDNA libraries that were hybridized on Human Gene 1.1ST arrays (Affymetrix) and analyzed with the Affymetrix Gene Chip Command Console Software v3.0 (AGCC 3.0, Affymetrix®) and the Expression Console Software v1.1 (Affymetrix®).
2014-09-09 | E-GEOD-50839 | biostudies-arrayexpress
Project description:Natural killer (NK) cells are a type of innate lymphocytes that play key roles in immune surveillance against tumors and viral infection. NK cells distinguish abnormal cells from healthy cells by cell-cell interaction with cell surface proteins and then attack target cells via multiple mechanisms involving TRAIL, Fas Ligand, cytokine secretion, perforin, and granzymes. In addition, extracellular vesicles (EVs), including exosomes derived from NK cells (NK-EVs), possess cytotoxic capacity against tumor cells, but their characteristics and regulation by cytokines remain unknown. Here, we report that EVs derived from human NK-92 cells stimulated with IL-15 + IL-21 show enhanced cytotoxic capacity against tumor cells in a granzyme B independent manner. In addition, small RNA-seq and mass spectrometry analyses indicate that miRNA and protein profiles in EVs are altered by cytokine stimulation. We also show NK-EVs are taken up by target cells via macropinocytosis. Collectively, our findings reveal novel characteristics of NK-EVs and the mechanism of their incorporation into target cells.
2021-09-09 | PXD018982 | Pride
Project description:Natural Killer (NK) cells present natural cytotoxicity against tumor cells, although their activity is increased after activation.NK cell activation depends on a complex intracellular signaling process mediated by activating and inhibitory receptors and the functional outcome depends on the integration of the activating and inhibitory signals received. Soluble cytokines and/or ligands on target cells bind the NK cell receptors, and hence, influence the final NK cell response: attack versus ignorance. We used microarrays to detail the global programme of gene expression underlying NK cell activation by IL-2, a MHC-I-deficient target cell (K562)+IL-2 and an EBV-target cell (R69).
2014-09-09 | GSE50839 | GEO
Project description:Exercise leads to a rapid change in the profile of gene expression in NK cells. We hypothesized that microRNA expression in circulating NK cells would also be affected by brief exercise. Eleven healthy men (20-29 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 77% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACSM-BM-. Pro Separator). Total RNA was extracted using TRIzolM-BM-.. For this study we used Agilent Human miRNA microarrays V2 (total of 22 chips).
2013-04-02 | E-GEOD-41913 | biostudies-arrayexpress
Project description:Nasal NK-cell lymphomas are EBV-infected lymphomas of the nasal mucosa. The genome-wide miRNA expression profiles of 36 cases of NNLs are determined with the NanoString technology. It is found that the expression levels of let-7 inhibits progression of nasal lymphomas.
2016-12-31 | GSE53479 | GEO
Project description:Exercise leads to a rapid change in the profile of gene expression in NK cells. We hypothesized that microRNA expression in circulating NK cells would also be affected by brief exercise.
2013-04-02 | GSE41913 | GEO
Project description:Human Immune Gene Expression Profile in activated and expanded NK cells, TIGITKO NK cells, GD2.CAR NK cells and TIGITKOGD2.CAR NK cells
| PRJNA1216524 | ENA
Project description:Expression data from stimulated NK cells
| PRJNA433730 | ENA