Project description:Random genomic sequencing and analysis by the Genolevures Consortium as part of a comparative genomics study of hemiascomycete yeasts
Project description:Random genomic sequencing and analysis by the Genolevures Consortium as part of a comparative genomics study of hemiascomycete yeasts
Project description:Random genomic sequencing and analysis by the Genolevures Consortium as part of a comparative genomics study of hemiascomycete yeasts
Project description:Random genomic sequencing and analysis by the Genolevures Consortium as part of a comparative genomics study of hemiascomycete yeasts
Project description:Oleaginous yeasts are valuable systems for biosustainable production of hydrocarbon-based chemicals. Yarrowia lipolytica is one of the best characterized of these yeast with respect to genome annotation and flux analysis of metabolic processes. Nonetheless, progress is hampered by a dearth of genomewide tools enabling functional genomics. The Hermes DNA transposon was expressed to achieve saturation mutagenesis of the Y. lipolytica genome. Over 535 thousand independent insertions were identified by next-generation sequencing. Poisson analysis of insertion density classified ~22% of genes as essential. As expected, most essential genes not only have homologues in Saccharomyces cerevisiae and Schizosaccharomyces pombe, but the majority of those are also essential. As an obligate aerobe, Y. lipolytica has significantly more respiration - related genes that are classified as essential than do S. cerevisiae and S. pombe. The findings provide insights into biosynthetic pathways, compartmentalization of enzymes, and distinct functions of paralogs. Contributions of nonessential genes to fitness were determined in log growth cultures with glucose and glycerol carbon sources. Fluorescence-activated cell sorting identified mutants in which lipid accumulation is increased. Biological contributions of genes to growth were used to evaluate two recent genome-scale models Y. lipolytica metabolism. This study is the first functional genomic analysis of an oleaginous yeast and provides an important resource for modeling and bioengineering of Y. lipolytica.
Project description:A comparison between the coculture of Debaryomyces hansenii with Yarrowia lipolytica and the monoculture of Debaryomyces hansenii, was carried out at two hours of culture (12h and 27h), in order to understand the metabolic changes induced in D. hansenii when grown in coculture with Y. lipolytica . The transcriptomic study was carried out using Agilent 8X15k slides with a custom oligoset designed by V. Loux using the Debaryomyces hansenii ORFeome extracted from genolevures database
Project description:Random genomic sequencing and analysis of strain CBS 94 by the Genolevures Consortium as part of a comparative genomics study of hemiascomycete yeasts
Project description:A comparison between Y. lipolytica growth in a Synthetic agar Medium not containing THiamin (SM TH-) to its growth in a Synthetic agar Medium supplemented with THiamin (SMTH+) at day 8 of the culture. This study allowed us to discover the effect of thiamin on the growth and the morphology of Y. lipolytica The transcriptomic study was carried out using Agilent 8X15k slides with a custom oligoset designed by V. Loux using the Yarrowia lipolytica ORFeome extracted from genolevures database
