Project description:Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue for Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4) treated with drought, heat at different air humidities, or combined drought-heat stress at different air humidities. This experiment contains FT-ICR-MS measurements for 103 Arabidopsis thaliana rosette leaf samples covering three genotypes under six different environmental conditions. The three genotypes comprise the Col-0 wildtype and two loss-of-function mutants of aquaporins, a pip2;1 pip2;2 double mutant and a pip2;1 pip2;2 pip2;4 triple mutant (respective AGI locus identifiers: AT3G53420, AT2G37170, AT5G60660). The six conditions include control condition (well-watered, 22 °C, 70% relative air humidity), drought stress (one week without watering), heat stress without changing the absolute humidity of the ambient air (6 hours at 33 °C, 37% relative air humidity), heat stress with supplemented air humidity to maintain a constant vapor pressure deficit before and during the heat episode (6 hours at 33 °C, 84% relative air humidity), and the combinations of drought pretreatment with each of the two heat stress variants (one week of drought followed by 6 hours of heat stress). Samples from all conditions were harvested at the same time (within 15 min starting at 5 pm). For validation, GC-TOF-MS measurements were done for two genotypes (wildtype, double mutant) and two conditions (drought, control) on partially overlapping samples.
Project description:By knocking out genes encoding specific arogenate dehydrase (ADT) enzymes in Arabidopsis, variable reductions in lignin can be achieved. To understand how ADT composition affects plant phenotypes and biomolecular systems, we successfully constructed single and multiple ADT knockout (KO) mutants in Arabidopsis. Using these mutants, a multi-omics (metabolome, transcriptome and proteome) evaluation was conducted using GC- and LC-MS, RNA-Seq, and iTRAQ labeled LC-MS/MS technologies. Identifications include primary and secondary metabolites, transcripts, and proteins in leaf and stem samples taken at 4 weeks of age from 9 KO and wild-type (WT) lines.
Project description:Transcriptional profiling of Arabidopsis thaliana Ler wildtype and eid3 (empfindlicher im dunkelroten Licht 3) mutant seedlings in darkness and 45 min after a red-light pulse. Arabidopsis thaliana Ler wildtype and eid3 mutant seedlings were grown on 1/2 MS Agar plates covered with filter paper for 4 days in darkness after induction of germination with 2 h red light. Samples were either treated with 2 min red light (30 µmol/m2s) or kept in darkness and harvested after additional 45 min in darkness. 3 biological replicas were used for each of the 4 experimental conditions.
Project description:Comparison of gene expression between shoots of root-wounded seedlings and shoots of control seedlings in Arabidopsis. We identified wounding-induced early (30 min) and late (360 min) root to shoot responsive genes (RtS).
Project description:Transcriptional profiling of fully developed leaves of Arabidopsis Col0 after 30 min of high-light stress (1000 umol*m-2*s-1). Goal was to find early transcriptional responses to immediate high-light stress, before acclimation responses appear.
Project description:Transcriptional profiling of Arabidopsis thaliana Ler wildtype and eid3 (empfindlicher im dunkelroten Licht 3) mutant seedlings in darkness and 45 min after a red-light pulse.
Project description:Gene expression changes were monitored in cultures of the cyanobacterium Synechocystis sp. PCC 6803 at 30 min after a shift from cultivation under standard light conditions (50 µmol photons m-2 s-1) to high light (HL; 250 µmol photons m-2 s-1) and 1 h after a shift from the standard growth temperature of 30°C to 20°C (cold). Acclimation to shifts in light and temperature is critical for photoautotrophic organisms such as cyanobacteria. The RRM domain proteins Rbp2 and Rpb3 are RNA-binding proteins that bind specific mRNAs and are involved in the localization of these mRNAs to the thylakoid membrane. Here, we investigated the effects of Rpb2 and Rbp3 on gene expression changes during the acclimation responses to an increase in light intensity and a decrease in temperature. We analyzed cultures of the wild-type (WT) and of the Δrbp2Δrbp3 double mutant, which lacks Rbp2 and Rbp3. We found that the transcript levels of psaA and psaB genes were significantly decreased in cells of the Δrbp2Δrbp3 double mutant upon cold stress and 30 min of high light compared to wild type. In addition, the transcript levels of Rbp1, another RRM domain-containing protein, were greatly increased.
