Project description:The goal of this study was to identify androgen regulated genes in the forebrain, prostate, ovary, and skin. The results of this study will be used to make comparisons between androgen regulation of gene expression in different androgen-receptor expressing tissues, and will contribute to our understanding of tissue-selective androgen activity. RNA was extracted from tissues castrate male rodents after 16 hours of treatment of either vehicle (Ctrl) or DHT. The 4 tissues of extraction are Forebrain (ForeB.Ctrl & ForeB.DHT), Ovary (Ovary.Ctrl & Ovary.DHT), Prostate (Prost.Ctrl & Prost.DHT), and Skin (Skin.Ctrl & Skin.DHT). There are 3 biological replicates per group for a total of 24 samples.
Project description:The goal of this study was to identify androgen regulated genes in the forebrain, prostate, ovary, and skin. The results of this study will be used to make comparisons between androgen regulation of gene expression in different androgen-receptor expressing tissues, and will contribute to our understanding of tissue-selective androgen activity.
Project description:Analysis of hormone effects on irradiated LBNF1 rat testes, which contain only somatic cells except for a few type A spermatgogonia. Rats were treated for 2 weeks with either sham treatment (group X), hormonal ablation (GnRH antagonist and the androgen receptor antagonist flutamide, group XAF), testosterone supplementation (GnRH antagonist and testosterone, group XAT), and FSH supplementation ((GnRH antagonist, androgen receptor antagonist, and FSH, group XAFF). Results provide insight into identifying genes in the somatic testis cells regulated by testosterone, LH, or FSH.
Project description:Analysis of LBNF1 rat testes from controls, containing both somatic and all germ cell types and from irradiated rats in which all cells germ cells except type A spermatgogonia are eliminated. Results provide insight into distinguishing germ and somatic cell genes and identification of somatic cell genes that are upregulated after irradiation.
Project description:In order to establish a rat embryonic stem cell transcriptome, mRNA from rESC cell line DAc8, the first male germline competent rat ESC line to be described and the first to be used to generate a knockout rat model was characterized using RNA sequencing (RNA-seq) analysis.
