Project description:RNA-seq of coding RNA in human fibroblasts, Peripheral Blood Mononuclear Cells (PBMCs) and induced pluripotent stem cells (iPS cells) as part of the HipSci project
Project description:Genome-Scale draft model for Human Peripheral Blood Mononuclear Cells (PBMCs). A GEM for PBMCs was developed by applying the INIT
algorithm on Human Metabolic Reconstruction (HMR 2.0) as a template model. GEMs were contextualised/ constrained for different conditions using expression datasets. The gene/transcript expression data obtained from PBMCs of Type 1 Diabetes progressors, non-progressors, and healthy controls were employed to score each reaction of HMR 2.0. For further detail please refer to Electronic Supplementary Information of Sen et.al, Metabolic alterations in immune cells associate with progression to type 1 diabetes, Diabetologia, 15/01/2020, (https://doi.org/10.1007/s00125-020-05107-6).
Project description:The gene expression profile of peripheral blood mononuclear cells (PBMCs) of infants (6 to 11 months) immunized with an inactivated EV71 vaccine or placebo