Project description:Genome-Scale draft model for Human Peripheral Blood Mononuclear Cells (PBMCs). A GEM for PBMCs was developed by applying the INIT
algorithm on Human Metabolic Reconstruction (HMR 2.0) as a template model. GEMs were contextualised/ constrained for different conditions using expression datasets. The gene/transcript expression data obtained from PBMCs of Type 1 Diabetes progressors, non-progressors, and healthy controls were employed to score each reaction of HMR 2.0. For further detail please refer to Electronic Supplementary Information of Sen et.al, Metabolic alterations in immune cells associate with progression to type 1 diabetes, Diabetologia, 15/01/2020, (https://doi.org/10.1007/s00125-020-05107-6).
Project description:Analysis of peripheral blood mononuclear cells (PBMCs) separated from whole blood of healthy male subjects - prior to onset of exercise - immediately following the end of exercise and - immediately following 1 hour of recovery from exercise Keywords: other
Project description:To investigate differential protein and phospho-protein changes in the signaling cascades related to mutant G2019S LRRK2 using peripheral blood mononuclear cells (PBMCs)
Project description:We found that peripheral blood mononuclear cells (PBMCs) (from subjects with allergy to nickel) stimulated with nickel were characterized by a specific miRNA signature that were different from vehicle-stimulated PBMCs.
Project description:Genome wise DNA methylation profiling of peripheral blood mononuclear cells (PBMCs) obtained from younger sedentary (Y-SED), older sedentary (O-SED) and older aerobically exercise trained (O-Ex) human subjects. The Illumina 450K methylation beadchip array was used to obtain DNA methylation profiles across approximately 450,000 CpG dinucleotide methylation loci in DNA isolated from PBMCs. Samples include 12 Y-SED subjects, 15 O-SED subjects and 11 O-Ex subjects.
Project description:Purified human ITPRIPL1-extracellular domain (ITPRIPL1-ECD) protein or PBS was added to human peripheral blood mononuclear cells (PBMCs) as a widely used source of immune cells and treated for 18 hours under 37℃, 5% CO2 in separate 1.5 ml EP tubes (n=3 independent samples for each condition)
Project description:Analysis of peripheral blood mononuclear cells (PBMCs) separated from whole blood of healthy male subjects; - prior to onset of exercise; - immediately following the end of exercise and; - immediately following 1 hour of recovery from exercise. To reduce individual variation and other confounding events, such as spontaneous up- and downregulation of genes, the 15 subjects in this study were pooled into 5 groups of 3 subjects each. The pools were not randomized; rather, we attempted to match the mean lymphocyte response to exercise across the five groups.
