Project description:In this study, we applied the isobaric tags for relative and absolute quantitation (iTRAQ) technique to detect alterations in the proteomic profile of the jejunal mucosa using a porcine model in which piglets were offered the protein-limited (PL) diet. Protein identification and quantification for iTRAQ experiments were performed using ProteinPilot (v4.0.8085) software. The LC-MS/MS data were searched against the UniProtKB (sus scrofa). To minimize the false discovery rate (FDR), a threshold for protein identification was applied, with the confident value > 95% (amount to the confident value “unused ProtScore” > 1.3 in ProteinPilot software), and at least one unique peptide was considered for protein identification. Proteins that were quantified with fold change > 2.0 were considered to be differentially expressed proteins. We identified 5275 proteins, 202 of which were differentially expressed. Furthermore, we adopted function annotation analysis of all identified proteins and function enrichment analysis of all differentially expressed proteins to explore more meaningful proteins and pathways.

2016-12-22 | PXD004069 | Pride

Sus scrofa

Project description:RNA-seq studies on the porcine model of heart donation after circulatory death

| PRJNA1002377 | ENA

Sus scrofa

Project description:PORCINE metagenome

| PRJNA309904 | ENA

Sus scrofa

Project description:Porcine liver

| PRJNA87687 | ENA

Sus scrofa

Project description:The porcine microRNA transcriptome response to porcine cytomegalovirus infection