Dataset Information

Sus scrofa (pig) small intestinal mucosa LC-MS/MS

ABSTRACT: In this study, we applied the isobaric tags for relative and absolute quantitation (iTRAQ) technique to detect alterations in the proteomic profile of the jejunal mucosa using a porcine model in which piglets were offered the protein-limited (PL) diet. Protein identification and quantification for iTRAQ experiments were performed using ProteinPilot (v4.0.8085) software. The LC-MS/MS data were searched against the UniProtKB (sus scrofa). To minimize the false discovery rate (FDR), a threshold for protein identification was applied, with the confident value > 95% (amount to the confident value “unused ProtScore” > 1.3 in ProteinPilot software), and at least one unique peptide was considered for protein identification. Proteins that were quantified with fold change > 2.0 were considered to be differentially expressed proteins. We identified 5275 proteins, 202 of which were differentially expressed. Furthermore, we adopted function annotation analysis of all identified proteins and function enrichment analysis of all differentially expressed proteins to explore more meaningful proteins and pathways.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Jejunal Mucosa

SUBMITTER: Jingdong Yin

LAB HEAD: Jingdong Yin, State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University

PROVIDER: PXD004069 | Pride | 2016-12-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Pig_CNM_6iTRAQ_f1.wiff	Wiff
	Pig_CNM_6iTRAQ_f1.wiff.scan	Wiff
	Pig_CNM_6iTRAQ_f10.wiff	Wiff
	Pig_CNM_6iTRAQ_f10.wiff.scan	Wiff
	Pig_CNM_6iTRAQ_f11.wiff	Wiff

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Publications

A proteomic adaptation of small intestinal mucosa in response to dietary protein limitation.

Qin Chunfu C Qiu Kai K Sun Wenjuan W Jiao Ning N Zhang Xin X Che Lianqiang L Zhao Haiyi H Shen Hexiao H Yin Jingdong J

Scientific reports 20161114

Dietary protein limitation (PL) is not only beneficial to human health but also applied to minimize nitrogen excretion in livestock production. However, the impact of PL on intestinal physiology is largely unknown. In this study, we identified 5275 quantitative proteins using a porcine model in which pigs suffered PL. A total of 202 proteins |log<sub>2</sub> fold-change| > 1 were taken as differentially expressed proteins and subjected to functional and pathway enrichment analysis to reveal prot ...[more]

PMID: 27841298

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