Project description:T lymphocytes are orchestrators of adaptive immunity. Naïve T cells may differentiate into the Th1, Th2, Th17 or iTreg phenotype, depending on environmental co-stimulatory signals. In order to identify the genes and pathways involved in differentiation of Jurkat T cells towards Th1 and Th2 subtypes we performed comprehensive transcriptome analyses of Jurkat T cells stimulated with various stimuli an pathway inhibitors Jurkat T cells were treated with CD3/CD28 and CD3/PMA and CD28/PMA. RNA was isolated after 1 and 8 hrs stumalation.
Project description:T lymphocytes are orchestrators of adaptive immunity. Naïve T cells may differentiate into the Th1, Th2, Th17 or iTreg phenotype, depending on environmental co-stimulatory signals. In order to identify the genes and pathways involved in differentiation of Jurkat T cells towards Th1 and Th2 subtypes we performed comprehensive transcriptome analyses of Jurkat T cells stimulated with various stimuli an pathway inhibitors Jurkat T cells were treated with CD3, CD28 and PMA and all pairwise combinations, in the presence of DMSO (control) or kinase inhibitors. RNA was isolated after 8 hrs incubation.
Project description:To broadly assess which pathways FRCs regulate in CD8+ T cells, we sorted CD8+ T cells for RNA-seq following activation in whole splenocyte mixtures via soluble anti-CD3/CD28 for 48 hours (Stim), activated with anti-CD3/CD28 in the presence of Nos2–/– FRCs (FRC) or activated with anti-CD3/CD28 plus 100 ng/ml recombinant IL-6 (IL-6). Here we demonstrate that FRC-derived signals, including IL-6, act in concert with TCR signaling and co-stimulation to promote the expression of MYC and HIF-1-dependent glycolytic genes and vital pro-survival genes (encoding BCL-2 family members, inhibitors of apoptosis [IAPs] members and Cflar) in activated CD8+ T cells.
Project description:To assess the effects of small molecules SP100030 and Selinexor on Jurkat T cells activated by PMA/Ionomycin after 6 hours of activation and treatment
Project description:In vitro culture of 2 separate sets of naive human T cells (from 2 adult male donors), activated using CD3 and CD28 antibodies. Secretome collection at 48 hours after activation. The same cell cultures not activated and sampled at the exact same time served as 'not-activated control'.
Project description:In vitro culture of 2 separate sets of naive human T cells (from 2 adult male donors), activated using CD3 and CD28 antibodies. Secretome collection at 48 hours after activation. The same cell cultures not activated and sampled at the exact same time served as 'not-activated control'.
2020-10-30 | MSV000086390 | MassIVE
Project description:PMA vs CD3/CD28 Bead Actiavation scRNAseq Comparison
Project description:This SuperSeries is composed of the following subset Series:; GSE10232: CTDG in PMA-activated Jurkat cells; GSE10233: CTDG in PMA-activated MM6 cells; GSE10234: CTDG in LPS-activated MM6 cells; GSE10737: CTDG in non-activated Jurkat cells; GSE10738: CTDG in non-activated MM6 cells; GSE10739: LPS and PMA response in parental MM6 cells Experiment Overall Design: Refer to individual Series
