Project description:T lymphocytes are orchestrators of adaptive immunity. Naïve T cells may differentiate into the Th1, Th2, Th17 or iTreg phenotype, depending on environmental co-stimulatory signals. In order to identify the genes and pathways involved in differentiation of Jurkat T cells towards Th1 and Th2 subtypes we performed comprehensive transcriptome analyses of Jurkat T cells stimulated with various stimuli an pathway inhibitors Jurkat T cells were treated with CD3/CD28 and CD3/PMA and CD28/PMA. RNA was isolated after 1 and 8 hrs stumalation.
Project description:T lymphocytes are orchestrators of adaptive immunity. Naïve T cells may differentiate into the Th1, Th2, Th17 or iTreg phenotype, depending on environmental co-stimulatory signals. In order to identify the genes and pathways involved in differentiation of Jurkat T cells towards Th1 and Th2 subtypes we performed comprehensive transcriptome analyses of Jurkat T cells stimulated with various stimuli an pathway inhibitors Jurkat T cells were treated with CD3, CD28 and PMA and all pairwise combinations, in the presence of DMSO (control) or kinase inhibitors. RNA was isolated after 8 hrs incubation.
Project description:To assess the effects of small molecules SP100030 and Selinexor on Jurkat T cells activated by PMA/Ionomycin after 6 hours of activation and treatment
Project description:In vitro culture of 2 separate sets of naive human T cells (from 2 adult male donors), activated using CD3 and CD28 antibodies. Secretome collection at 48 hours after activation. The same cell cultures not activated and sampled at the exact same time served as 'not-activated control'.
Project description:In vitro culture of 2 separate sets of naive human T cells (from 2 adult male donors), activated using CD3 and CD28 antibodies. Secretome collection at 48 hours after activation. The same cell cultures not activated and sampled at the exact same time served as 'not-activated control'.
