Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA146375 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene expression profiling of ElonginA knocked-down Hela cells in the presence of Doxorubicin
| PRJNA143535 | ENA
Project description:Gene expression profiles of MCF7 with CARM1 knocked down
| PRJNA135097 | ENA
Project description:To identify differentially up or downregulated genes in MCF7_ADR cell compare to MCF7, we have employed whole genome microarray expression profiling. We used microarray to evaluate the up or down regulated genes in doxorubicin resistant MCF7_ADR cells compare to MCF7
2016-10-13 | GSE87864 | GEO
Project description:To evaluate whether PLXDC2 mediated the role of PTEN on macrophages, PLXDC2 was knocked down in M2-polarized BMDMs, and the resultant PLXDC2-knocked down cells together with control cells were treated with or without PTEN, before subjected to global transcriptome profiling.
2024-07-25 | GSE249266 | GEO
Project description:The purpose of the study was to identify mRNA bound to HuR in the presence of doxorubicin in MCF7 cells. We collected cytoplasmic RNA from untreated and treated cells and detected differentially expressed genes (DEGs). We also coimmunoprecipitated HuR and IgG (as control) from doxorubicin treated cells. Comparison between HuR RIP and IgG RIP signals was used to discriminate specific mRNA bound to HuR. HuR coimmmunoprecipitated material was hybridized together with cytoplasmic mRNA of doxorubicin treated cells, enabling the fold enrichment calculation and the selection of mRNAs bound to HuR. Keywords: RIP-Chip, HuR, doxorubicin, MCF7, HuR consensus binding, post-transcriptional regulation. We subjected MCF7 cells to starvation for 24h and then we added doxorubicin at final concentration of 10 uM, profiling before and after 4 hours of treatment in biological quadruplicate (only on cytoplasmic mRNAs, as HuR was found in the cytoplasm). Differentially expressed genes, altered during the treatment, were identified. Data derived from HuR RIP-Chip and IgG RIP-Chip (in biological quadruplicate) allowed the identification of specific mRNAs bound to HuR. The comparison between HuR RIP-Chip and cytoplasmic extracts from doxorubicin treated cells (in biological triplicate) identified those genes that were more strictly bound to HuR independently from their expression levels.
2011-12-31 | E-GEOD-33055 | biostudies-arrayexpress
Project description:Gene Expression Pattern in EP300 knocked-down MCF7 cells and corresponding paclitaxel resistant derivatives
| PRJNA306618 | ENA
Project description:Gene expression profiling of SKOV-3 cells with or without PDK1 knocked down (Control versus siPDK1)
| PRJNA592533 | ENA
Project description:Gene Expression Profiling Of TTLL12 Knocked Down Cell With Sendai Virus Treatment
| PRJNA327204 | ENA
Project description:The effects of several compounds on the MCF7 human adenocarcinoma mammary cell line were analysed by gene expression profiling. Tested compounds: HSP90 inhibitors: 17AAG (Tanespimycin), NVP-AUY922, NMS-E973 (cpd developed at NMS). CDK inhibitors: CDK-887 (cpd developed at NMS). Topoisomerase inhibitors: Doxorubicin, SN38 (active metabolite of Irinotecan). The MCF7 cell line was treated with the different compounds for 6 hours at a dose equal to 5 times the IC50. Untreated MCF7 cells were used as a control. Two replicates per treatment.
2010-07-14 | E-GEOD-19638 | biostudies-arrayexpress
Project description:Transcriptional elongation factor Elongin A exhibits an activity to repress the temporary pause of RNA polymerase II (RNAPII) on the DNA template, resulting in increase of mRNA synthesis in vitro. However, actual elongation ability of Elongin A in mammalian cells has not been determined yet. To elucidate the practical transcriptional role of mammalian Elongin A in vivo, we carried out whole genome expression analysis using Elongin A knocked-down HeLa cells. Gene expression in Elongin A knocked-down HeLa cells was measured at 2 hours after doxorubicin treatment (1microM).
2013-09-03 | E-GEOD-30432 | biostudies-arrayexpress