Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA147531 | ENA |
REPOSITORIES: ENA
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Project description:We focus our analyses on the description of viral small RNAs (FHVdeltaB2 or FHV) and based on genetic and molecular evidende, classify them and discuss their relevance in antiviral defense. Small RNAs from adult Drosophila flies from different genetic backgrounds and infected with FHV-deltaB2 or FHV were sequenced using the illumina platform.
2011-11-12 | E-GEOD-32011 | biostudies-arrayexpress
Project description:<p>Viral studies of Drosophila melanogaster typically involve virus injection with a small needle, causing post-injury a wounding/wound healing response, in addition to the effects of viral infection. However, the metabolic response to the needle injury is understudied, and many viral investigations neglect potential effects of this response. Furthermore, the wMel strain of the endosymbiont bacterium Wolbachia pipientis provides anti-viral protection in Drosophila. Here we used NMR-based metabolomics to characterise the acute wounding response in Drosophila and the relationship between wound healing and the Wolbachia strain wMel. The most notable response to wounding was found on the initial day of injury and lessened with time in both uninfected and Wolbachia infected flies. Metabolic changes in injured flies revealed evidence of inflammation, Warburg-like metabolism and the melanisation immune response as a response to wounding. In addition, at five days post injury Wolbachia infected injured flies were metabolically more similar to the uninjured flies than uninfected injured flies were at the same time point, indicating a positive interaction between Wolbachia infection and wound healing. This study is the first metabolomic characterisation of the wound response in Drosophila and its findings are crucial to the metabolic interpretation of viral experiments in Drosophila in both past and future studies.</p>
2025-11-18 | MTBLS13248 | MetaboLights
Project description:small RNAs of Dmel infected or not by FHV or circular-viral-DNA of FHV
| PRJNA387406 | ENA
Project description:The fruit fly Drosophila melanogaster is a good model to unravel the molecular mechanisms of innate immunity, and has led to some important discoveries on the sensing and signalling of microbial infections. The response of drosophila to virus infections remains poorly characterized, and appears to involve two facets. On one hand RNA interference (RNAi) involves the recognition and processing of dsRNA into small interfering (si) RNAs by the host ribonuclease Dicer-2 (Dcr-2), whereas on the other hand an inducible response controlled by the evolutionarily conserved JAK/STAT pathway contributes to the antiviral host defence. In order to clarify the contribution of the siRNA and JAK/STAT pathways to the control of viral infections, we have compared the resistance of flies wild-type or mutant for Dcr-2 or the JAK kinase Hopscotch (Hop) to infections by seven RNA or DNA viruses belonging to different families. Our results reveal a unique susceptibility of hop mutant flies to infection by DCV and CrPV, two members of the Dicistroviridae family. Genome-wide microarray analysis confirmed that different sets of genes were induced following infection by DCV (GSE2828) or two unrelated RNA viruses, FHV and SINV. Overall, our data reveal that RNAi is an efficient antiviral mechanism, operating against a large range of viruses, including a DNA virus. By contrast, the antiviral contribution of the JAK/STAT pathway appears to be virus-specific. For each experimental challenge (FHV, 48 or 72 hours after infection; SINV, 4 or 8 days after infection), three biologically independent samples composed of 45 male Oregon R flies were used. Infection has been performed by injecting viral stocks prepared in Tris solution. Injection of the same volume of Tris has been used as control. Infected flies were then incubated for 48 or 72 hours in the case of FHV and 4 days or 8 days in the case of SINV.
2013-02-06 | E-GEOD-31542 | biostudies-arrayexpress
Project description:Drosophila melanogaster small RNAs in ovaries
| PRJNA215819 | ENA
Project description:We focus our analyses on the description of viral small RNAs (FHVdeltaB2 or FHV) and based on genetic and molecular evidende, classify them and discuss their relevance in antiviral defense.
2011-11-12 | GSE32011 | GEO
Project description:circular DNA of Drosophila melanogaster S2 cells infected or not by FHV
| PRJNA387403 | ENA
Project description:Small RNAs from third instar Drosophila melanogaster males
| PRJNA262549 | ENA
Project description:High throughput seqeuncing of small RNAs (PAGE isolated from total RNA or Argonaute immunoprecipitates) from Drosophila melanogaster using the Illumina platform. Adapter ligation requires 5' monophosphate and 3' OH. Full analysis of all libraries in this set is published (Czech B. et al. 2008), leading to the description of endogenous siRNAs in flies. Keywords: Solexa sequences
2008-04-30 | GSE11086 | GEO
Project description:Drosophila melanogaster DNA genomic sequencing and small RNAs sequencing
| PRJNA104947 | ENA