Cupriavidus metallidurans CH34
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ABSTRACT:
PROVIDER: PRJNA148575 | ENA |
REPOSITORIES: ENA
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Project description:Various environmental bacteria were adapted to the presence of toxic and recalcitrant organic solvents. Cupriavidus metallidurans CH34, a β-proteobacterium that was found in industrial environments is known as a bacterial model to study heavy metal resistance. Interestingly, genome screening of CH34 also reveals the existence of genes involved in the degradation of recalcitrant organic solvents, such acetone and aromatic compounds. Here, we showed that this bacterium could resist a large variety of organic solvents, and was also able to metabolize some of them. In particular, investigations were focused on acetone and isopropanol catabolism. Integrative studies based on transcriptomic (DNA microarrays), proteomic (2D-DIGE and Isotope-Coded Protein Label technology) and biochemical analyses (enzyme purification and characterization) showed a similar catabolic pathway for both molecules which involved the AcxABC acetone carboxylase. First, isopropanol is oxidized into acetone by the Adh alcohol dehydrogenase. Acetoacetate production from acetone is then catalyzed by the acetone carboxylase. The generated acetoacetate molecules are then transformed by the PacIJ 3-oxoacid CoA-transferase, to acetoacetyl-CoA and succinate. Finally, an acetyl-CoA acetyltransferase catalyses the hydrolysis of acetoacetyl-CoA into 2 acetyl-CoA that are introduced into the glyoxylate cycle. As demonstrated, key enzymes of the acetone/isopropanol catabolism (encoded by acx and ald genes) are under the control of a σ54-dependent RNA polymerase. Moreover, the catabolic pathway involved in acetone and isopropanol consumption was repressed when gluconate was given as alternative carbon substrate, displaying a new example of diauxie. The results presented here provide a comprehensive picture of acetone and isopropanol biodegradation in Cupriavidus metallidurans CH34 and strongly support the fact that CH34 can be considered as a solvent tolerant bacterium.
2015-08-31 | GSE33578 | GEO
Project description:Various environmental bacteria were adapted to the presence of toxic and recalcitrant organic solvents. Cupriavidus metallidurans CH34, a β-proteobacterium that was found in industrial environments is known as a bacterial model to study heavy metal resistance. Interestingly, genome screening of CH34 also reveals the existence of genes involved in the degradation of recalcitrant organic solvents, such acetone and aromatic compounds. Here, we showed that this bacterium could resist a large variety of organic solvents, and was also able to metabolize some of them. In particular, investigations were focused on acetone and isopropanol catabolism. Integrative studies based on transcriptomic (DNA microarrays), proteomic (2D-DIGE and Isotope-Coded Protein Label technology) and biochemical analyses (enzyme purification and characterization) showed a similar catabolic pathway for both molecules which involved the AcxABC acetone carboxylase. First, isopropanol is oxidized into acetone by the Adh alcohol dehydrogenase. Acetoacetate production from acetone is then catalyzed by the acetone carboxylase. The generated acetoacetate molecules are then transformed by the PacIJ 3-oxoacid CoA-transferase, to acetoacetyl-CoA and succinate. Finally, an acetyl-CoA acetyltransferase catalyses the hydrolysis of acetoacetyl-CoA into 2 acetyl-CoA that are introduced into the glyoxylate cycle. As demonstrated, key enzymes of the acetone/isopropanol catabolism (encoded by acx and ald genes) are under the control of a σ54-dependent RNA polymerase. Moreover, the catabolic pathway involved in acetone and isopropanol consumption was repressed when gluconate was given as alternative carbon substrate, displaying a new example of diauxie. The results presented here provide a comprehensive picture of acetone and isopropanol biodegradation in Cupriavidus metallidurans CH34 and strongly support the fact that CH34 can be considered as a solvent tolerant bacterium. Two-condition experiments. Comparing samples after induction with acetone versus non-induced samples. Biological triplicate. Each array contains 3 technical replicates.
2015-08-31 | E-GEOD-33578 | biostudies-arrayexpress
Project description:Metal resistant bacterium Cupriavidus metallidurans CH34 uses non-conventional proteins to combat oxidative stress Keywords: Cupriavidus metallidurans; hydrogen peroxide; oxidative stress;
2014-09-13 | GSE24094 | GEO
Project description:Response of Cupriavidus metallidurans CH34 to cisplatin, Pt(IV)chloride and Au-NP
2016-02-27 | GSE78723 | GEO
Project description:Response of Cupriavidus metallidurans CH34 to NaAuCl4, Au(I)-thiomalate, Au(I)-thiosulfate, KAuCN, (KCN as control)
2013-03-15 | GSE42199 | GEO
Project description:Metal resistant bacterium Cupriavidus metallidurans CH34 uses non-conventional proteins to combat oxidative stress Keywords: Cupriavidus metallidurans; hydrogen peroxide; oxidative stress; Two-condition experiments. Comparing samples after challenging with oxidative stress versus non-induced samples. Biological triplicate. Each array contains 3 technical replicates.
2014-09-13 | E-GEOD-24094 | biostudies-arrayexpress
Project description:Response of Cupriavidus metallidurans CH34 to cisplatin, Pt(IV)chloride and Au-NP In this study 7 different treatments were performed (first 2 as 3 replicates) to acquire expression profiles of the total genome of Cupriavidus metallidurans
2016-02-27 | E-GEOD-78723 | biostudies-arrayexpress
Project description:Response of Cupriavidus metallidurans CH34 to NaAuCl4, Au(I)-thiomalate, Au(I)-thiosulfate, KAuCN, (KCN as control) In this study 10 different treatments were performed (first 7 as 3 replicates) to acquire expression profiles of the total genome of Cupriavidus metallidurans
2013-03-15 | E-GEOD-42199 | biostudies-arrayexpress
Project description:Proteomic response of Cupriavidus metallidurans CH34 to gold stress as in comparison to copper stress and control. Please note that 25Au in gel file names is a typo and should read 50Au.
2016-10-26 | PXD005034 | Pride
Project description:Cupriavidus metallidurans CH34 is a metal resistant beta-proteobacterium. The genome of this bacterium contain many genes involved in heavy metal resistance. Gene expression of C. metallidurans was studied after the addition of of Zn(II), Cd(II), Cu(II), Ni(II), Pb(II), Hg(II) or Co(II). Keywords: Heavy metal stress response
2007-03-16 | GSE7272 | GEO