Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA149323 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Deep proteome of HeLa, A549, HCT116, MCF7, and SHSY5Y cell lines
2021-05-25 | E-PROT-19 | ExpressionAtlas
Project description:We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We report that the majority of miRNA genes encode mature isomers that vary in size by one or more bases at the 3’ and/or 5’ end of the miRNA. Northern blotting for individual miRNAs showed that the proportions of isomiRs expressed by a single miRNA gene often differ between cell and tissue types. IsomiRs were readily co-immunoprecipitated with Argonaute proteins in vivo and were active in luciferase assays, indicating that they are functional. Bioinformatics analysis predicts substantial differences in targeting between miRNAs with minor 5’ differences and in support of this we report that a 5’ isomiR-9-1 gained the ability to inhibit the expression of DNMT3B and NCAM2 but lost the ability to inhibit CDH1 in vitro. This result was confirmed by the use of isomiR-specific sponges. Our analysis of the miRGator database indicates that a small percentage of human miRNA genes express isomiRs as the dominant transcript in certain cell types and analysis of miRBase shows that 5’ isomiRs have replaced canonical miRNAs many times during evolution. This strongly indicates that isomiRs are of functional importance and have contributed to the evolution of miRNA genes
2014-08-06 | GSE58734 | GEO
Project description:The permanent reduction of TIA proteins molds expression transcriptome in HeLa cells.
2025-01-24 | E-GEOD-47664 | ExpressionAtlas
Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.
2019-07-31 | GSE114179 | GEO
Project description:Expression data from docetaxel-resistant prostate cancer cell lines
2025-01-24 | E-GEOD-33455 | ExpressionAtlas
Project description:Gene expression from H69M versus H69 SCLC cell lines
2025-01-24 | E-GEOD-45120 | ExpressionAtlas
Project description:Expression profiling in four human bladder cancer cell lines
2025-01-23 | E-MTAB-2610 | ExpressionAtlas
Project description:Global expression analysis of DLBCL cell lines with LITAF over-expression or silencing
2025-01-24 | E-GEOD-42174 | ExpressionAtlas
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:Gene expression profile of two Ara-C-resistant cell lines
2015-09-07 | E-GEOD-18322 | ExpressionAtlas