ABSTRACT: Gene expression response in avian enterocytes cells isolated from genetically resistant and susceptible chickens infected with Salmonella Enteritidis.
Project description:H5N1 subtype highly pathogenic avian influenza virus has been spreading to Asia, Eurasia and African coutries. An original or six of recombinant H5N1 subtype influenza viruses with varying survivability were infected to chickens for elucidating genes correlated with pathogenicity.
Project description:White leghorn layers were infected with Salmonella Enteritidis. The cecum were collected at 7 days post infection for total RNA isolation. The significantly expressed microRNAs between infected and non-infected chickens were identified through Solexa sequencing technology.
Project description:H5N1 subtype highly pathogenic avian influenza virus has been spreading to Asia, Eurasia and African coutries. An original or six of recombinant H5N1 subtype influenza viruses with varying survivability were infected to chickens for elucidating genes correlated with pathogenicity. Two chickens were infected with each 10^6EID50/ head virus intranasally, and their lung was collected from infected chicken at 24 hours after infection.
Project description:Poultry products are an important source of Salmonella enterica. An effective way to reduce food poisoning due to Salmonella would be to breed chickens more resistant to Salmonella. Unfortunately resistance to Salmonella is a complex trait with many factors involved. To learn more about Salmonella resistance mechanisms in young chickens, a cDNA microarray analysis was performed to compare gene expression profiles between a Salmonella susceptible and a more resistant chicken line. Newly hatched chickens were orally infected with S. enterica serovar Enteritidis. Since the intestine is the first barrier the bacteria encountersbacteria encounter after oral inoculation, gene expression was investigated in the intestine, from day 1 until day 21 post infection. Differences in gene expression between the susceptible and resistant chicken line were found in control as well as Salmonella infected conditions. In response to the Salmonella infection, the expression of different sets of genes seemed to be affected in the jejunum of the two chicken lines. In the susceptible line this included genes that affect T-cell activation, whereas in the more resistant line, at day 1, macrophage activation seemed to be more affected. At day 7 and 9 most gene expression differences between the two chicken lines were identified under control conditions, indicating a difference in the intestinal development between the two chicken lines which might be linked to the difference in Salmonella susceptibility. The findings in this study have lead to the identification of novel genes and possible cellular pathways of the host involved in Salmonella susceptibility. Keywords: timecourse, disease
Project description:We sequenced single cells with the 10x platform of chicken spleens from chickens genetically resistant and genetically susceptible to Marek's disease, with both infected individuals and uninfected controls from both lines
Project description:In this study, we analyzed the global gene expression of enterocytes during carrier-state in chicken lines N and 61 which are respectively resistant and susceptible to Salmonella carrier-state. Transcriptome analysis was performed at three weeks post-inoculation, on purified enterocytes, by comparing the expression profiles of infected and control chicks in the two lines. Three weeks post-inoculation correspond to the period where bacterial load starts to decrease in the resistant line but is still high in the susceptible line; this point could conceptually corresponds to genes which are differentially regulated leading to the resistant or susceptible phenotypes. Functional annotation of differentially expressed genes allowed us to identify numerous biological processes modulated during Salmonella carriage. Keywords: Expression profiling by array For each chicken line, twenty slides including ten biological replicates and the corresponding dye-swaps with the fluorophores reversed were carried out on control and infected animals.
Project description:In this study, we analyzed the global gene expression of enterocytes during carrier-state in chicken lines N and 61 which are respectively resistant and susceptible to Salmonella carrier-state. Transcriptome analysis was performed at three weeks post-inoculation, on purified enterocytes, by comparing the expression profiles of infected and control chicks in the two lines. Three weeks post-inoculation correspond to the period where bacterial load starts to decrease in the resistant line but is still high in the susceptible line; this point could conceptually corresponds to genes which are differentially regulated leading to the resistant or susceptible phenotypes. Functional annotation of differentially expressed genes allowed us to identify numerous biological processes modulated during Salmonella carriage. Keywords: Expression profiling by array
Project description:Relative expression levels of mRNAs in chicken cecal epithelia experimentally infected with Eimeria tenella were measured at 4.5 days post-infection. Two weeks old chickens were uninfected (negative control) or were orally inoculated with sporulated oocysts of Eimeria tenella. Cecal epithelia samples were collected from >12 birds in infected or uninfected group at 4.5 d following infections, in which samples from 4 birds were pooled together to form a total 3 biological replicates in each group. Parasite merozoites were also collected from four infected chickens at 5 d after infections. Uninfected control samples, merozoites and infection group samples were selected for RNA extraction and hybridization on Affymetrix microarrays. We used Affymetrix GeneChip chicken genome arrays to detail the chicken cecal epithelia gene expression in the control and E. tenella-infected birds.
Project description:1-day old chickens were orally inoculated with 1e5 c.f.u. Salmonella enteritidis. 24 hours after infection RNA from the jejunum was analysed. Two different microarray platforms were used, a homemade cDNA microarray and the Affymetrix GeneChip Chicken Genome Array Keywords: host response; genetic background
