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Gene expression response in avian enterocytes cells isolated from genetically resistant and susceptible chickens infected with Salmonella Enteritidis.

ABSTRACT: In this study, we analyzed the global gene expression of enterocytes during carrier-state in chicken lines N and 61 which are respectively resistant and susceptible to Salmonella carrier-state. Transcriptome analysis was performed at three weeks post-inoculation, on purified enterocytes, by comparing the expression profiles of infected and control chicks in the two lines. Three weeks post-inoculation correspond to the period where bacterial load starts to decrease in the resistant line but is still high in the susceptible line; this point could conceptually corresponds to genes which are differentially regulated leading to the resistant or susceptible phenotypes. Functional annotation of differentially expressed genes allowed us to identify numerous biological processes modulated during Salmonella carriage. Keywords: Expression profiling by array For each chicken line, twenty slides including ten biological replicates and the corresponding dye-swaps with the fluorophores reversed were carried out on control and infected animals.

ORGANISM(S): Gallus gallus

SUBMITTER: Olivier GrÃ©pinet

PROVIDER: E-GEOD-34580 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression response in avian enterocytes cells isolated from genetically resistant and susceptible chickens infected with Salmonella Enteritidis.

Project description:In this study, we analyzed the global gene expression of enterocytes during carrier-state in chicken lines N and 61 which are respectively resistant and susceptible to Salmonella carrier-state. Transcriptome analysis was performed at three weeks post-inoculation, on purified enterocytes, by comparing the expression profiles of infected and control chicks in the two lines. Three weeks post-inoculation correspond to the period where bacterial load starts to decrease in the resistant line but is still high in the susceptible line; this point could conceptually corresponds to genes which are differentially regulated leading to the resistant or susceptible phenotypes. Functional annotation of differentially expressed genes allowed us to identify numerous biological processes modulated during Salmonella carriage. Keywords: Expression profiling by array

2014-06-01 | GSE34580 | GEO

Identification of Novel Virulence-related Genes in Burkholderia pseudomallei by Comparing Gene Expression Profiles in Two Mice Model Through Microarray

Project description:The gene expression profiles were investigated and compared in spleen, lungs and liver of susceptible (BALB/c) and resistant (C57BL/6) hosts to identify genes participated in survival and virulence functions encoded in the B. pseudomallei genome. Subsequently, genes with unknown function and located with virulence gene clusters or suspected to be transcription regulator were selected for a novel virulence gene investigation DNA microarray was used to compare gene expression profiles of B. pseudomallei 1909a in spleen, lung and liver of infection-susceptible BALB/c mice (Th2 prototype) and infection-resistant C57BL/6 mice, (Th1 prototype) during an acute infection. The gene expression profile of B. pseudomallei in culture medium at mid-log phase was used to identify genes that expressed higher or lower in vivo when compared to the in vitro gene profile KEYWORDS: Gene expression

2014-09-12 | E-GEOD-51369 | biostudies-arrayexpress

Gene expression responses to a Salmonella infection in the chicken intestine differ between lines.

Project description:Poultry products are an important source of Salmonella enterica. An effective way to reduce food poisoning due to Salmonella would be to breed chickens more resistant to Salmonella. Unfortunately resistance to Salmonella is a complex trait with many factors involved. To learn more about Salmonella resistance mechanisms in young chickens, a cDNA microarray analysis was performed to compare gene expression profiles between a Salmonella susceptible and a more resistant chicken line. Newly hatched chickens were orally infected with S. enterica serovar Enteritidis. Since the intestine is the first barrier the bacteria encountersbacteria encounter after oral inoculation, gene expression was investigated in the intestine, from day 1 until day 21 post infection. Differences in gene expression between the susceptible and resistant chicken line were found in control as well as Salmonella infected conditions. In response to the Salmonella infection, the expression of different sets of genes seemed to be affected in the jejunum of the two chicken lines. In the susceptible line this included genes that affect T-cell activation, whereas in the more resistant line, at day 1, macrophage activation seemed to be more affected. At day 7 and 9 most gene expression differences between the two chicken lines were identified under control conditions, indicating a difference in the intestinal development between the two chicken lines which might be linked to the difference in Salmonella susceptibility. The findings in this study have lead to the identification of novel genes and possible cellular pathways of the host involved in Salmonella susceptibility. Keywords: timecourse, disease

2005-12-05 | GSE3066 | GEO

Metal resistant bacterium Cupriavidus metallidurans CH34

Project description:Metal resistant bacterium Cupriavidus metallidurans CH34 uses non-conventional proteins to combat oxidative stress Keywords: Cupriavidus metallidurans; hydrogen peroxide; oxidative stress; Two-condition experiments. Comparing samples after challenging with oxidative stress versus non-induced samples. Biological triplicate. Each array contains 3 technical replicates.

2014-09-13 | E-GEOD-24094 | biostudies-arrayexpress

Gallus gallus

Project description:Gene expression response in avian enterocytes cells isolated from genetically resistant and susceptible chickens infected with Salmonella Enteritidis.

| PRJNA151009 | ENA

Project description:Colistin resistant and colistin susceptible Salmonella enterica

| PRJNA594836 | ENA

Microarray of IVM susceptable and resistant Haemonchus contortous

Project description:Anthelmintic resistance is a major problem in the global fight against parasitic nematodes., Most previous studies have focused on the analysis of potential candidate genes that may have a role in resistance. Here we take a novel approach in the important parasite, Haemonchus contortus, by investigating changes in microRNA expression between resistant and susceptible parasites. The resistant worms included two geographically distinct strains and two lines generated by multiple rounds of backcrossing between susceptible and resistant parents, with ivermectin selection. All four resistant strains showed increased abundance of a single miRNA, hco-miR-9551, compared to the susceptible strain. hco-miR-9551 is enriched in female worms, is likely to be located on the X chromosome and is found only in clade V parasitic nematodes. Approximately 5-10% of the genomes of the resistant parental strains are introgressed into the respective backcrosses, suggesting that hco-miR-9551, or genes regulated by the miRNA, may be genetically linked to a locus that determines resistance. Genes containing predicted binding sites for hco-miR-9551 were identified computationally and refined based on differential expression in a transcriptomic dataset. These findings advance our conceptual understanding of the molecular mechanisms of anthelmintic resistance in H. contortus and indicate that altered miRNA expression may be linked with drug resistance. All samples were carried out using three biological replicates

2016-03-25 | E-GEOD-79542 | biostudies-arrayexpress

Gene expression profiling to identify uterine chicken proteins associated with the supply of minerals and potentially actives on the biomineralisation process during eggshell formation

Project description:We used a transcriptomic approach based on the comparison of the expression in the uterine tissue when an hard-shelled egg was in calcification (normal) versus uterine tissue collected after premature expulsion of the egg before mineralization has begun (expelled) to better appreciate which uterine chicken proteins are associated with the supply of minerals and potentially actives on the biomineralisation process during eggshell formation. A total of 16 microarray slides were used for hybridization of the 8 uterine tissues collected when an hard-shelled egg was in calcification (normal), and mixed to the 8 uterine tissues collected after premature expulsion of the egg before mineralization has begun (expelled) were analysed, with an experimental design in dye swap (all the slides labeled with fluorophore Alexa 555 and with Alexa 647 for each condition).

2013-12-10 | E-GEOD-52823 | biostudies-arrayexpress

Transcriptional response of an azole-resistant Candida parapsilosis isolate [voriconazole].

Project description:Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of voriconazole. Whole genome microarrays were used to compare the transcriptional response of the voriconizole-resistant and susceptible isolates. Transcriptional profile of an in vitro derived voriconazole-resistant isolate of C. parapsilosis (BC014VRC) compared to the susceptible isolate (BC014S). Cell were grown in YPD medium in normoxia at 35 degrees. Each strain was labelled with Cy3 or Cy5. Overall, 4 independent biological replicates were compared; 2 dye swaps were performed to normalize dye effects.

2011-04-18 | E-GEOD-27408 | biostudies-arrayexpress

Transcriptional response of an azole-resistant Candida parapsilosis isolate [fluconazole].

Project description:Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of fluconazole. Whole genome microarrays were used to compare the transcriptional response of the fluconazole-resistant and susceptible isolates. Transcriptional profile of in vitro derived fluconazole resistant isolate of C. parapsilosis (BC014FLC) compared to susceptible isolate (BC014S). Cell were grown in YPD medium in normoxia at 35 degrees. Each strain was labelled with Cy3 or Cy5. Overall, 4 independent biological replicates were compared; 2 dye swaps were performed to normalize dye effects.

2011-04-18 | E-GEOD-27405 | biostudies-arrayexpress

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