Project description:An eQTL analysis between DBA/2J and AKR/J mice strains on the ApoE null background was done in bone marrow derived macrophages and endothelial cells to identify modifier genes that affect atherosclerosis susceptibility. DBA/2J mice were crossed with AKR/J mice and their offspring crossed with each other to yield an F2 generation. Bone marrow derived macrophages were cultured and gene expression quantified using Illumina's MouseRef-8 v2 arrays in 170 F2 mice for an eQTL study. In addition primary endothelial cells derived from the aorta of 48 male F2 mice were also assayed using arrays for eQTL mapping. Lesion area in the aortic root was also quantified in these samples.
Project description:An eQTL analysis between DBA/2J and AKR/J mice strains on the ApoE null background was done in bone marrow derived macrophages and endothelial cells to identify modifier genes that affect atherosclerosis susceptibility.
Project description:Bone marrow-derived macrophages from mice were treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
Project description:Second dataset release from the Immunological Proteome Resource (ImmPRes). This dataset contains both lymphoid and myeloid populations. The whole list is as follows: Bone marrow derived eosinophils, bone marrow derived mast cells, bone marrow derived macrophages, NK cells, TH2 cells, TH17 cells, Cytotoxic T cells cultured in IL15 and Naive CD8+ T cells extractred from the lymph nodes of C57BL/6J mice.
Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
