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ABSTRACT: GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors

PROVIDER: PRJNA152625 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR402845.fastq.gz	Fastqsanger.gz
	SRR402846.fastq.gz	Fastqsanger.gz
	SRR402847.fastq.gz	Fastqsanger.gz
	SRR402848.fastq.gz	Fastqsanger.gz
	SRR402849.fastq.gz	Fastqsanger.gz

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GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors

Project description:This SuperSeries is composed of the following subset Series: GSE35379: Genome-wide occupancy map of GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors (ES-EP) GSE35384: Transcriptome analysis of differentiating normal and leukemic erythroid progenitors Refer to individual Series

2012-02-27 | E-GEOD-35385 | biostudies-arrayexpress

Genome-wide occupancy map of GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors (ES-EP)

Project description:We find GATA-1 occupies 6,600 sites in proliferating erythroid progenitors and 10,600 sites in differentiating progenitors. 80-90% of GATA-1 binds within intragenic or intergenic regions, while <20% of GATA-1 is found within 2kb of TSS. Assaying GATA-1 occupancy in normal erythroid progenitors in both proliferating and differentiating conditions.

2012-02-27 | E-GEOD-35379 | biostudies-arrayexpress

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Project description:Genome-wide occupancy map of GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors (ES-EP)

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Transcriptome analysis of differentiating normal and leukemic erythroid progenitors

2012-02-27 | GSE35384 | GEO

Genome-wide occupancy map of GATA-1 in proliferating and differentiating murine ES cell derived erythroid progenitors (ES-EP)

2012-02-27 | GSE35379 | GEO

Transcriptome analysis of normal erythroid progenitors and erythroleukemia cells

Project description:We compared the transcriptomes of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with Gata-1 fused to ER. RNA was isolated from duplicate proliferating cultures of MEL and ES-EP using Affymetrix GeneChip Mouse Gene 1.0 ST.

2011-06-23 | E-GEOD-21949 | biostudies-arrayexpress

Transcriptome analysis of differentiating normal and leukemic erythroid progenitors

Project description:We compared the transcriptomes of differentiating cultures of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with GATA-1 fused to ER. RNA was isolated from duplicate differntiating cultures of MEL and ES-EP using Affymetrix GeneChip Mouse Gene 1.0 ST.

2012-02-27 | E-GEOD-35384 | biostudies-arrayexpress

Transcriptome analysis of normal erythroid progenitors and erythroleukemia cells

Project description:We compared the transcriptomes of ES cell derived erythroid progentor cells (ES-EP) and murine erythroleukemia (MEL) cells stably transfected with Gata-1 fused to ER.

2011-06-23 | GSE21949 | GEO

Inducible Gata1 Suppression Expands Erythro-Megakaryocytic Progenitors from Embryonic Stem Cells

Project description:Embryonic stem (ES) and induced pluripotent stem (iPS) cells represent a potential source of megakaryocytes and platelets for transfusion therapies. However, most current ES/iPS cell differentiation protocols are limited by low yields of hematopoietic progeny. Mutations in the mouse and human genes encoding transcription factor GATA1 cause accumulation of proliferating, developmentally arrested megakaryocytes. To exploit this clinical observation, we engineered wildtype (WT) murine ES cells to express doxycycline (dox)-regulated Gata1 short hairpin (sh) RNAs. In vitro differentiation with dox and thrombopoietin (Tpo) resulted in approximately 1013-fold expansion of immature hematopoietic progenitors. Upon dox withdrawal with multilineage cytokines, GATA1 expression was restored and the cells differentiated into erythroblasts and megakaryocytes. With Tpo alone, dox-deprived progenitors formed mainly mature megakaryocytes that generated functional platelets in vivo. Our findings provide a novel, readily reproducible strategy to expand ES-cell derived megakaryocyte-erythroid progenitors and direct their differentiation into megakaryocytes producing functional platelets in clinically relevant numbers. 3 classes of samples were compared 1) fetal liver derived megkaryocytes 2) G1ME (Gata1â megakaryocyte-erythroid) 3) G1ME2 (engineered wildtype (WT) murine ES cells to express doxycycline (dox)-regulated Gata1 short hairpin (sh) RNAs)

2015-12-09 | E-GEOD-66479 | biostudies-arrayexpress

Inducible Gata1 Suppression Expands Erythro-Megakaryocytic Progenitors from Embryonic Stem Cells

2015-12-09 | GSE66479 | GEO