Homo sapiens
Ontology highlight
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PROVIDER: PRJNA152897 | ENA |
REPOSITORIES: ENA
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Project description:Uterine leiomyoma is the most common benign tumor of the female genital tract, and is the main cause of hysterectomy in 25-30% of affected women. Nevertheless, knowledge about stem cells initiating these common uterine tumors remains scarce. The side population method has been used to identify different somatic stem cells in the human body. In this context, our study explores the hypothesis that human leiomyoma side population cells could be the putative somatic stem cells responsible for leiomyoma's initiation and formation. We isolated, identified and characterized the side population cells from human leiomyomas which implies no commitment to the myometrial lineage at the molecular level, differential cloning efficiency under hypoxic conditions and provides a leiomyoma stem cells gene profile. Based on their cloning efficiency ability, we also established two cell lines (MyoSP1-2) with a normal karyotype under hypoxic conditions. The phenotype analysis supported their mesodermal commitment as assessed by the positive expression of typical mesenchymal markers, such as CD90, CD105, and CD73, and by the absence of hematopoietic stem cell markers like CD34 and CD45. At the mRNA level, we also confirmed their undifferentiated status (OCT-4+, NANOG+, DNMT3B+, GDF3+) and mesenchymal lineage commitment as demonstrated by their ability to differentiate in vitro into adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of Myo cell lines (MyoSP1-2) to form human leiomyoma-like tissue after injecting this subset of cells either under the kidney capsule or in the subcutaneous tissue in the NOD-SCID mice model. 4 replicates each of leiomyoma side population (SP) cells and leiomyoma total fraction (FT) cells were analyzed.
2012-08-14 | E-GEOD-36157 | biostudies-arrayexpress
Project description:Uterine leiomyoma is the most common benign tumor of the female genital tract, and is the main cause of hysterectomy in 25-30% of affected women. Nevertheless, knowledge about stem cells initiating these common uterine tumors remains scarce. The side population method has been used to identify different somatic stem cells in the human body. In this context, our study explores the hypothesis that human leiomyoma side population cells could be the putative somatic stem cells responsible for leiomyoma's initiation and formation. We isolated, identified and characterized the side population cells from human leiomyomas which implies no commitment to the myometrial lineage at the molecular level, differential cloning efficiency under hypoxic conditions and provides a leiomyoma stem cells gene profile. Based on their cloning efficiency ability, we also established two cell lines (MyoSP1-2) with a normal karyotype under hypoxic conditions. The phenotype analysis supported their mesodermal commitment as assessed by the positive expression of typical mesenchymal markers, such as CD90, CD105, and CD73, and by the absence of hematopoietic stem cell markers like CD34 and CD45. At the mRNA level, we also confirmed their undifferentiated status (OCT-4+, NANOG+, DNMT3B+, GDF3+) and mesenchymal lineage commitment as demonstrated by their ability to differentiate in vitro into adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of Myo cell lines (MyoSP1-2) to form human leiomyoma-like tissue after injecting this subset of cells either under the kidney capsule or in the subcutaneous tissue in the NOD-SCID mice model.
2012-08-15 | GSE36157 | GEO
Project description:Proteomic characterization of human hepatocellular carcinoma (HCC) tumor biopsies
2024-06-05 | E-PROT-142 | ExpressionAtlas
Project description:CAL-51 breast cancer side population cells
2025-01-24 | E-GEOD-18773 | ExpressionAtlas
Project description:Identification and Initial Functional Characterization of SENCR, a Long Non-Coding RNA Enriched in Human Vascular Cells
2025-01-24 | E-GEOD-51878 | ExpressionAtlas
Project description:Identification of WISP1 as an important survival factor in human mesenchymal stem cells
2025-01-24 | E-GEOD-37603 | ExpressionAtlas
Project description:The recent identification of cancer stem cells (CSCs) in multiple human cancers provides a new inroad to understanding tumorigenesis at the cellular level. CSCs are defined by their characteristics of self-renewal, multipotentiality, and tumor initiation upon transplantation. By testing for these defining characteristics, we provide evidence for the existence of CSCs in a transgenic mouse model of glioma, S100Ã-verbB;Trp53. In this glioma model, CSCs are enriched in the side-population (SP) cells. These SP cells have enhanced tumor-initiating capacity, self-renewal, and multipotentiality compared to non-SP cells from the same tumors. Furthermore, gene expression analysis comparing FACS-sorted cancer SP cells to non-SP cancer cells and normal neural SP cells identified 45 candidate genes that are differentially expressed in glioma stem cells. We validated the expression of two genes from this list (S100a4 and S100a6) in primary mouse gliomas and human glioma samples. Analyses of xenografted human GBM (glioblatoma multiforme) cell lines and primary human glioma tissues show that S100A4 and S100A6 are expressed in a small subset of cancer cells and that their abundance is positively correlated to tumor grade. In conclusion, this study shows that CSCs exist in a mouse glioma model, suggesting that this model can be used to study the molecular and cellular characteristics of CSCs in vivo and to further test the cancer stem cell hypothesis. Experiment Overall Design: This study features two factors, injected cell origin (either tumorsphere or neurosphere) and FACS cell population (either side population or non-side population cells). There were two different tumorspheres, labeled 3447 and 4346 that were isolated from brain tumors in S100beta-verbB;p53-/- or S100beta-verbB;p53+/- mice. The tumorspheres were injected separately into the brains of NOD.Cg-Prkdc<scid>Il2rg<tm1Wjl>/SzJ mice to generate biological triplicates of each primary tumor. Tumorspheres were isolated and cultured before FACS sorting to obtain side population and non-side population cells. As a control, untransformed neurospheres from three independent S100beta-verbB;p53-/- or S100beta-verbB;p53+/- mice were isolated, cultured, and FACS sorted to obtain side population and non-side population cells. Side population and non-side population cells cultured from three mice injected with the 3447 cultured tumorspheres were assayed for gene expression (six samples). Side-population stem cells cultured from three mice injected with the 4346 cultured tumorspheres were assayed for gene expression (three samples). Side-population and non-side population cells cultured from three mice injected with the neurospheres were assayed for gene expression (six samples).
2009-01-13 | E-GEOD-13490 | biostudies-arrayexpress
Project description:Expression data from undifferentiated human embryonic stem cells (hESC) and Day 3.5 mesodermal progenitor (CD326neg CD56+) population
2025-01-24 | E-GEOD-21668 | ExpressionAtlas
Project description:Gene expression of side population and main population cells, isolated from adult murine skeletal muscle and bone marrow
2015-09-03 | E-GEOD-1571 | ExpressionAtlas
Project description:Expression data from side-population sorted putative intestinal stem cells.
| PRJNA102501 | ENA