Homo sapiens
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PROVIDER: PRJNA152897 | ENA |
REPOSITORIES: ENA
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Project description:Uterine leiomyoma is the most common benign tumor of the female genital tract, and is the main cause of hysterectomy in 25-30% of affected women. Nevertheless, knowledge about stem cells initiating these common uterine tumors remains scarce. The side population method has been used to identify different somatic stem cells in the human body. In this context, our study explores the hypothesis that human leiomyoma side population cells could be the putative somatic stem cells responsible for leiomyoma's initiation and formation. We isolated, identified and characterized the side population cells from human leiomyomas which implies no commitment to the myometrial lineage at the molecular level, differential cloning efficiency under hypoxic conditions and provides a leiomyoma stem cells gene profile. Based on their cloning efficiency ability, we also established two cell lines (MyoSP1-2) with a normal karyotype under hypoxic conditions. The phenotype analysis supported their mesodermal commitment as assessed by the positive expression of typical mesenchymal markers, such as CD90, CD105, and CD73, and by the absence of hematopoietic stem cell markers like CD34 and CD45. At the mRNA level, we also confirmed their undifferentiated status (OCT-4+, NANOG+, DNMT3B+, GDF3+) and mesenchymal lineage commitment as demonstrated by their ability to differentiate in vitro into adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of Myo cell lines (MyoSP1-2) to form human leiomyoma-like tissue after injecting this subset of cells either under the kidney capsule or in the subcutaneous tissue in the NOD-SCID mice model. 4 replicates each of leiomyoma side population (SP) cells and leiomyoma total fraction (FT) cells were analyzed.
2012-08-14 | E-GEOD-36157 | biostudies-arrayexpress
Project description:Uterine leiomyoma is the most common benign tumor of the female genital tract, and is the main cause of hysterectomy in 25-30% of affected women. Nevertheless, knowledge about stem cells initiating these common uterine tumors remains scarce. The side population method has been used to identify different somatic stem cells in the human body. In this context, our study explores the hypothesis that human leiomyoma side population cells could be the putative somatic stem cells responsible for leiomyoma's initiation and formation. We isolated, identified and characterized the side population cells from human leiomyomas which implies no commitment to the myometrial lineage at the molecular level, differential cloning efficiency under hypoxic conditions and provides a leiomyoma stem cells gene profile. Based on their cloning efficiency ability, we also established two cell lines (MyoSP1-2) with a normal karyotype under hypoxic conditions. The phenotype analysis supported their mesodermal commitment as assessed by the positive expression of typical mesenchymal markers, such as CD90, CD105, and CD73, and by the absence of hematopoietic stem cell markers like CD34 and CD45. At the mRNA level, we also confirmed their undifferentiated status (OCT-4+, NANOG+, DNMT3B+, GDF3+) and mesenchymal lineage commitment as demonstrated by their ability to differentiate in vitro into adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of Myo cell lines (MyoSP1-2) to form human leiomyoma-like tissue after injecting this subset of cells either under the kidney capsule or in the subcutaneous tissue in the NOD-SCID mice model.
2012-08-15 | GSE36157 | GEO
Project description:The recent identification of cancer stem cells (CSCs) in multiple human cancers provides a new inroad to understanding tumorigenesis at the cellular level. CSCs are defined by their characteristics of self-renewal, multipotentiality, and tumor initiation upon transplantation. By testing for these defining characteristics, we provide evidence for the existence of CSCs in a transgenic mouse model of glioma, S100Ã-verbB;Trp53. In this glioma model, CSCs are enriched in the side-population (SP) cells. These SP cells have enhanced tumor-initiating capacity, self-renewal, and multipotentiality compared to non-SP cells from the same tumors. Furthermore, gene expression analysis comparing FACS-sorted cancer SP cells to non-SP cancer cells and normal neural SP cells identified 45 candidate genes that are differentially expressed in glioma stem cells. We validated the expression of two genes from this list (S100a4 and S100a6) in primary mouse gliomas and human glioma samples. Analyses of xenografted human GBM (glioblatoma multiforme) cell lines and primary human glioma tissues show that S100A4 and S100A6 are expressed in a small subset of cancer cells and that their abundance is positively correlated to tumor grade. In conclusion, this study shows that CSCs exist in a mouse glioma model, suggesting that this model can be used to study the molecular and cellular characteristics of CSCs in vivo and to further test the cancer stem cell hypothesis. Experiment Overall Design: This study features two factors, injected cell origin (either tumorsphere or neurosphere) and FACS cell population (either side population or non-side population cells). There were two different tumorspheres, labeled 3447 and 4346 that were isolated from brain tumors in S100beta-verbB;p53-/- or S100beta-verbB;p53+/- mice. The tumorspheres were injected separately into the brains of NOD.Cg-Prkdc<scid>Il2rg<tm1Wjl>/SzJ mice to generate biological triplicates of each primary tumor. Tumorspheres were isolated and cultured before FACS sorting to obtain side population and non-side population cells. As a control, untransformed neurospheres from three independent S100beta-verbB;p53-/- or S100beta-verbB;p53+/- mice were isolated, cultured, and FACS sorted to obtain side population and non-side population cells. Side population and non-side population cells cultured from three mice injected with the 3447 cultured tumorspheres were assayed for gene expression (six samples). Side-population stem cells cultured from three mice injected with the 4346 cultured tumorspheres were assayed for gene expression (three samples). Side-population and non-side population cells cultured from three mice injected with the neurospheres were assayed for gene expression (six samples).
2009-01-13 | E-GEOD-13490 | biostudies-arrayexpress
Project description:Expression data from side-population sorted putative intestinal stem cells.
| PRJNA102501 | ENA
Project description:Cancer stem cells have been strongly linked to resistance and relapse in many malignancies. However, purifying them from within the bulk tumor has been challenging, so their precise genetic and functional characteristics are not well defined. The side population assay exploits the ability of some cells to efflux Hoechst dye via ABC-transporters. Stem cells have increased expression of these transporters and this assay has been shown to enrich for stem cells in various tissues and cancers. This study identifies the side population within a zebrafish model of acute lymphoblastic leukemia and correlates the frequency of side population cells with the frequency of leukemia stem cells (more precisely referred to as leukemia propagating cells within our transplantation model). In addition, the side population within the leukemia evolves with serial transplantation, increasing in tandem with leukemia propagating cell frequency over subsequent generations. Sorted side population cells from these tumors are enriched for leukemia propagating cells and have enhanced engraftment compared to sorted non-side population cells when transplanted into syngeneic recipients. RNA-sequencing analysis of sorted side population cells compared to non-side population cells identified a shared expression profile within the side population and pathway analysis yielded Wnt-signaling as the most overrepresented. Gene set enrichment analysis showed that stem cell differentiation and canonical Wnt-signaling were significantly upregulated in the side population. Overall, these results demonstrate that the side population in zebrafish acute lymphoblastic leukemia significantly enriches for leukemia propagating cells and identifies the Wnt-pathway as a likely genetic driver of leukemia stem cell fate.
2021-12-10 | GSE123665 | GEO
Project description:Cancer stem-like cells are defined as small population of cancer cells which has, higher tumor-initiating ability, self-renewing ability and differentiation ability. In this experiment, transcription profile of cancer stem-like cells derived from human colon cancer line cell SW480 was investigated. Cancer stem-like cells were isolated as side population (SP) cells by Hoechst33342 dye staining from human colon cancer line cell SW480. Non-cancer stem-like cells were isolated as main population (MP) cells. RNAs were isolated from SP cells and MP cells derived from SW480 cells and array experiment was perfromed in dye swaped fashion.
2015-05-31 | E-MTAB-2605 | biostudies-arrayexpress
Project description:Immature cell populations, including stem cells and progenitor cells, can be found in “side-population (SP)” cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP cells remained to be reported. We used microarrays to detail the global program of gene expression underlying cell differentiation and identified up-regulated genes of SP cells.
2011-03-12 | GSE27909 | GEO
Project description:Immature cell populations, including stem cells and progenitor cells, can be found in “side-population (SP)” cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP cells remained to be reported. We used microarrays to detail the global program of gene expression underlying cell differentiation and identified up-regulated genes of SP
2014-09-18 | GSE61547 | GEO
Project description:While the existence of intestinal epithelial stem cells (IESCs) has been well established, their study has been limited due to the inability to isolate them. Previous work has utilized side population (SP) sorting of the murine small intestinal mucosa to isolate a viable fraction of cells enriched for putative IESCs. We have used microarray analyses to characterize the molecular features of this potential stem cell population. Keywords: Comparitive gene expression analysis
2007-11-12 | GSE9013 | GEO