Project description:This SuperSeries is composed of the following subset Series: GSE29791: Identification of putative targets of Nkx2-5 in Xenopus laevis using gene expression analysis and cross-species annotation GSE29795: Gene expression profile for X.laevis embryo head vs.tail Refer to individual Series
Project description:Purpose: Study the developing neural cell fate of the D1 blastomere in the 8-cell embryo. Determine what transcripts are dependant on cell/cell signaling and which are inherit to the single cell and do not depend on the rest of the embryo. Methods: D1 blastomere was either injected with fluorescent dye or explanted away from the developing embryo. Transcript profile was taken at 7 time points: 8-cell, 16-cell, 32-cell, blastula, gastrula, early neurula, and mid/late neurula. 3 biological triplicates were taken at each time point for both the dissect and explant condition. Results: Up until blastula stage, dissect and explant transcript profiles remain fairly similar. But following this the profiles completely diverge, with the dissect tissue heading towards the neural fate while the explant samples head towards an ectoderm/epithelial fate.
Project description:Proteome analysis of blastocoel fluid recovered from Xenopus laevis embryo was carried out with highly sensitive nanoLC-MS/MS system using miniaturized spray columns.
