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Klebsiella pneumoniae subsp. pneumoniae MGH 78578

ABSTRACT: Genome-wide identification of RNA polymerase, RpoD, and RpoS binding sites in Klebsiella pneumoniae MGH 78578

PROVIDER: PRJNA155703 | ENA |

REPOSITORIES: ENA

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Genome-wide identification of RNA polymerase, RpoD, and RpoS binding sites in Klebsiella pneumoniae MGH 78578

Project description:Genome-wide identification of RNA polymerase (RNAP) binding sites were performed in Klebsiella pneumoniae MGH 78578 (KP). Anti-RNAP is used to capture the RNAP in KP. ChIP-chip was performed on tiling array specifically made for KP.

2012-12-31 | GSE35927 | GEO

Genome-wide identification of RNA polymerase, RpoD, and RpoS binding sites in Klebsiella pneumoniae MGH 78578

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Expression analysis of Klebsiella pneumoniae MGH 78578 and capsule deletion mutant

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Alanine scan of potential linear epitopes of Klebsiella pneumoniae MGH 78578

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Genome-wide gene expression analysis for Klebsiella pneumoniae MGH 78578

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Screening for novel immungenic proteins of Klebsiella pneumoniae MGH 78578

Project description:The screening of a cDNA derived expression library of Klebsiella pneumoniae MGH 78578 expressed in E.coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. Thus, 1536 different clones were screened including positive (ompA, mdh) and negative (pyrC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from K. pneumoniae by selecting clones showing a high signal intensity in comparison to the known antigens used as positve markers. Afterwards, the most promising clones were sequenced to identify the gene and corresponding protein and these proteins were then investigated further. Consequently, 14 novel immunogenic proteins could be identified.

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Klebsiella pneumoniae subsp. pneumoniae MGH 78578

Project description:Genome-wide gene expression analysis for Klebsiella pneumoniae MGH 78578

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Klebsiella pneumoniae subsp. pneumoniae MGH 78578

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