Project description:This SuperSeries is composed of the following subset Series: GSE34050: ChIP-chip from DAOY cells stably expressing HA-MXD3 with anti-HA GSE34100: Expression profiling of MXD3 stable cell lines Refer to individual Series
Project description:Gene expression profiling of DAOY stable cell lines expressing a tamoxifen inducible MXD3. Data was normalized to a null version of MXD3 (unable to bind DNA). Goal of the study was to identify candidate genes regulated by MXD3.
Project description:NPAC ChIP were performed by anti-Flag and anti-HA tandem affinity purification from HeLa stably expressing Flag-HA tagged NPAC from pOZ-N vector, and enrichement on chromosome 3, 21, and 22 were determined by chip microarray analysis using Affymatrix HumanTiling 2.0 arrays
Project description:A subset of medulloblastomas, the most common brain tumor in children, is hypothesized to originate from granule neuron precursors (GNPs) in which the sonic hedgehog (SHH) pathway is over-activated. MXD3, a basic helix-look-helix zipper transcription factor of the MAD family, has been reported to be upregulated during postnatal cerebellar development and to promote GNP proliferation and MYCN expression. Mxd3 is upregulated in mouse models of medulloblastoma as well as in human medulloblastomas. Therefore, we hypothesize that MXD3 plays a role in the cellular events that lead to medulloblastoma biogenesis. The purpose of this study was to identify MXD3 target genes. Stable cell line overexpressing HA tagged MXD3 immunoprecipitated with anti-HA; 2 biological replicates on 2 promoter arrays
Project description:The HA-tagged G3BP1 complex was obtained by co-IP with anti-HA antibodies from UMUC3 cells stably expressing HA-tagged G3BP1. The purified immunoprecipitants were resolved on gradient SDS-PAGE, silver-stained, and subjected to MS analysis.
Project description:A subset of medulloblastomas, the most common brain tumor in children, is hypothesized to originate from granule neuron precursors (GNPs) in which the sonic hedgehog (SHH) pathway is over-activated. MXD3, a basic helix-look-helix zipper transcription factor of the MAD family, has been reported to be upregulated during postnatal cerebellar development and to promote GNP proliferation and MYCN expression. Mxd3 is upregulated in mouse models of medulloblastoma as well as in human medulloblastomas. Therefore, we hypothesize that MXD3 plays a role in the cellular events that lead to medulloblastoma biogenesis. The purpose of this study was to identify changes in gene expression in response to MXD3. RNA from MXD3 stable cell lines compared to vector-only cell lines and to parental (DAOY) cell line.
Project description:To determine interactors of Aurora-A, HA tagged Aurora-A was immunoprecipitated from MV4-11 cells stably expressing HA tagged Aurora-A wild type and compared to MV4-11 cells expressing empty vector.
