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The interactome of G3BP1 in UMUC3 cells

ABSTRACT: The HA-tagged G3BP1 complex was obtained by co-IP with anti-HA antibodies from UMUC3 cells stably expressing HA-tagged G3BP1. The purified immunoprecipitants were resolved on gradient SDS-PAGE, silver-stained, and subjected to MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Bladder Urothelial Cell

DISEASE(S): Bladder Transitional Cell Papilloma

SUBMITTER: Jiawei Chen

LAB HEAD: Jiawei Chen

PROVIDER: PXD035722 | Pride | 2024-05-23

REPOSITORIES: Pride

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			Action	DRS
	R20210901912_UMUC_G3IP.msf	Msf
	R20210901912_UMUC_G3IP.raw	Raw
	R20210901912_UMUC_IGG.msf	Msf
	R20210901912_UMUC_IGG.raw	Raw

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Publications

G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer.

Zheng Xianchong X Chen Jiawei J Deng Minhua M Ning Kang K Peng Yulu Y Liu Zhenhua Z Li Xiangdong X Zhou Zhaohui Z Tang Huancheng H Li Yaoying Y Kang Tiebang T Liu Zhuowei Z

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20231212 7

Immune checkpoint inhibitors (ICIs) show promise as second-line treatment for advanced bladder cancer (BLCA); however, their responsiveness is limited by the immune evasion mechanisms in tumor cells. This study conduct a Cox regression analysis to screen mRNA-binding proteins and reveals an association between Ras GTPase-activating protein-binding protein 1 (G3BP1) and diminished effectiveness of ICI therapy in patients with advanced BLCA. Subsequent investigation demonstrates that G3BP1 enhance ...[more]

PMID: 38084438

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G3BP1 Binds to Guanine Quadruplexes in mRNAs

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ChIP-seq analysis of G3BP1 in KGN cells

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G3BP1 inhibits Cul3SPOP to amplify AR signaling and promote prostate tumorigenesis

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RAN directly binds and stabilizes G3BP1 mRNA in the nucleus to facilitate proliferation and metastasis of nasopharyngeal carcinoma (si-G3BP1 RNA-seq)

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