Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming

ABSTRACT: In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming

PROVIDER: PRJNA159423 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming (miRNA)

Project description:Remarkable progress has been made in cell fate reprogramming by forced expression of a small number of transcription factors. Major challenges remain, however, in regenerative medicine regarding how to target multiple cell types and direct them to form a functional organ in vivo. Here, we demonstrate that, by changing their stromal microenvironment, adult differentiated cells of endodermal origin can be reprogrammed to generate a functional ectodermal organ. The process of organ regeneration is highly efficient and complete, and depends on epithelial-stromal interactions that lead to successful remodeling of the extracellular matrix and stromal cells that are essential for organ function. Furthermore, it is a multistep process consisting of changes in cell fate, where dedifferentiation occurs more rapidly than redifferentiation, and subsequent morphogenetic reprogramming. Remarkably, neither direct transdifferentiation nor a complete reversion to the pluripotency state is involved in the reprogramming process; instead it features dynamic activities of essential genes that regulate pluripotency and lineage development. Our data have important implications for stem cell biology, cancer biology, and regenerative medicine.

2021-12-31 | GSE37308 | GEO

In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming (mRNA)

2021-12-31 | GSE37289 | GEO

In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-31 | GSE37309 | GEO

Hyperinnervation inhibits organ-level regeneration in mammalian skin

Project description:Some mammalian tissues can replace lost cells within one lineage, but organ-level regeneration—restoring diverse cell types across lineages—remains rare. Here we show that late embryonic full-thickness skin injuries heal by regenerating epithelial, mesenchymal, neuronal, and vascular lineages with proper connectivity. However, this ability is lost soon after birth, leading to a failure to restore most cell types and hyperinnervation of the wound bed. Single-cell sequencing identified a postnatal wound-specific fibroblast (PWF) population absent after embryonic wounding. Through an in vivo screen, we discovered three PWF-enriched genes—Timp1, Cxcl12, and Ccl7—that inhibit organ-level regeneration and cause hyperinnervation when overexpressed in embryonic wounds. Reducing hyperinnervation by depleting Cxcl12 in fibroblasts or inhibiting synaptic release enables postnatal skin to regenerate diverse lineages after injury. Our study identifies mechanisms transitioning an organ from regenerative to non-regenerative, discovers fibroblast-driven hyperinnervation as a key barrier, and demonstrates removing this barrier unlocks organ-level regeneration.

2026-03-20 | GSE276119 | GEO

Lineage and Organ Signals Sequentially Build Organ Intrinsic Nervous Systems [Spatial Transcriptomics]

Project description:Lineage and Organ Signals Sequentially Build Organ Intrinsic Nervous Systems [Spatial Transcriptomics]

| PRJNA1431443 | ENA

Lineage and Organ Signals Sequentially Build Organ Intrinsic Nervous Systems [scRNA-seq]

Project description:Lineage and Organ Signals Sequentially Build Organ Intrinsic Nervous Systems [scRNA-seq]

| PRJNA1431444 | ENA

Hyperinnervation inhibits organ-level regeneration in mammalian skin

Project description:Hyperinnervation inhibits organ-level regeneration in mammalian skin

| PRJNA1154938 | ENA

Hedgehog gradient in the stroma maintains niche diversity and organ function

Project description:Hedgehog gradient in the stroma maintains niche diversity and organ function

| PRJNA398101 | ENA

Mus musculus

Project description:In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming (mRNA)

| PRJNA159473 | ENA

Mus musculus

Project description:In Vivo Organ Regeneration via Stroma-Dependent Adult Lineage Reprogramming (miRNA)

| PRJNA159475 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data