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Hedgehog gradient in the stroma maintains niche diversity and organ function

ABSTRACT: Hedgehog gradient in the stroma maintains niche diversity and organ function

PROVIDER: PRJNA398101 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR5930150_1.fastq.gz	Fastqsanger.gz
	SRR5930150_2.fastq.gz	Fastqsanger.gz
	SRR5930151_1.fastq.gz	Fastqsanger.gz
	SRR5930151_2.fastq.gz	Fastqsanger.gz

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Project description:A single-cell transcriptional analysis was performed on GLI2+ stromal cells from the adult murine lung. Whole adult murine lung tissue was dissociated to single cells and subjected to fluorescence activated cell sorting (FACS) to select all live GLI2+ cells. The single cell RNA-sequencing library was then subsequently generated. Cells were sequenced at a depth of ~60,000 reads/cell. We captured approximately 4,600 cells with a median of 2,246 genes detected per cell utilizing a droplet-based barcoding approach to capture single cells for RNA sequencing. The results demonstrate that the GLI2+ population is segregated into distinct stromal subtypes along the proximal-distal axis of the lung with distinct functional contribution to matrix production and paracrine signaling within their distinctive niche.

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