Project description:Expression data of Arabidopsis thaliana (Ler accession) phytochrome phyABCDE quintuple mutant and phyABDE quadruple mutant in response to red light, and their comparison to WT expression data
Project description:We used microarrays to detail the response of A. thaliana WT and gnat2 to high light and darkness treatments and identified distinct classes of regulated genes during this process. In wildtype, the expression of 1,808 of 27,826 analysed transcripts was significantly (>2-fold, p<0.05) altered after high light treatment. Of those 1,808 transcripts, 943 were up- and 865 were downregulated. A similar response to high light was observed in gnat2, where 2,083 transcripts (1,126 up and 957 down) were significantly regulated upon high light stress. Similarly, lack of light resulted in an altered expression of 2,042 transcripts. Of those 2,042 transcripts, 979 were up- and 1,063 were downregulated. The gnat2 mutant responded similarly with 2,361 transcripts (1,195 up and 1,166 down) significantly regulated upon darkness. Under control conditions, only 38 transcripts except GNAT2 were differentially regulated between WT and gnat2. Overall the transcriptional responses of the WT and gnat2 to unexpected short-term darkness and high light were similar.
Project description:In order to elucidate the role of the Arabidopsis thaliana LLM-domain B-GATAs in response to high light intensities, a transcriptomic analysis of Col-0, a hexuple LLM-domain B-GATA mutant hex (gnc gnl gata15 gata16 gata17 gata17l) and GNLox under high-ligh stress conditions was performed.
Project description:Nitrogen and light are two major regulators of plant metabolism and development. While genes involved in the control of each of these signals have begun to be identified, regulators that integrate gene responses to nitrogen and light signals have yet to be determined. Here, we evaluate the role of bZIP1, a transcription factor involved in light and nitrogen sensing, by exposing wild-type (WT) and bZIP1 T-DNA null mutant plants to a combinatorial space of N and L treatment conditions. We use ANOVA analysis combined with clustering and Boolean modeling, to evaluate the role of bZIP1 in mediating L and N signaling genome-wide.
