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Salmonella enterica subsp. enterica serovar Typhimurium

ABSTRACT: Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes.

PROVIDER: PRJNA168358 | ENA |

REPOSITORIES: ENA

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Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes.

Project description:The regulatory role of the Fis protein in fis and in the transcription of several gene regions during mid-exponential and late-stationary phase, and during different growth aeration regimes, has been investigated. Studies were done during those two growth phases and in aerated and non-aerated (microaerobic) conditions, to measure Fis enrichment and binding peaks in strategic gene regions by genome-wide microarray analysis ChIP-chip. This research investigation points to central roles for SPI-1, SPI-2, DNA gyrase and topoisomerase I, the elements of the stringent response, and the regulatory function of Fis-binding patterns, in setting and re-setting the activity of the fis gene and other involved promoters as a function of the growth conditions and aeration regimes experienced by Salmonella.

2013-05-01 | GSE38661 | GEO

ChIP-chip analysis of CspC binding in Salmonella enterica serovar Typhimurium strain SL1344

2015-02-26 | GSE66288 | GEO

Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes.

2013-05-01 | E-GEOD-38661 | biostudies-arrayexpress

ChIP-chip analysis of CspC binding in Salmonella enterica serovar Typhimurium strain SL1344

Project description:We performed Chromatin Immunoprecipitation (ChIP) and microarray hybridization analysis of CspC binding in Salmonella Typhimurium strain SL1344 which has been genetically engineered to express a 3xFLAG tagged CspC protein. Chromatin samples were prepared from SL1344 CspC 3xFLAG grown to exponential phase (OD600nm = 0.2). CspC FLAG ChIP and mock normal mouse IgG ChIP reactions were carried out. The purified ChIP DNA samples were hybridized to SL1344 tiling microarrays.

2015-02-26 | E-GEOD-66288 | biostudies-arrayexpress

FabR ChIP-chip on Salmonella enterica subsp. enterica serovar Typhimurium SL1344

Project description:FabR ChIP-chip on Salmonella enterica subsp. enterica serovar Typhimurium SL1344 using anti-Myc antibody against strain with chromosomally 9Myc-tagged FabR (IP samples) and wildtype strain (mock IP samples)

2014-12-31 | GSE52877 | GEO

Identification of Sfh and H-NS binding sites in the Salmonella Typhimurium genome

Project description:The global regulator H-NS represses transcription in gram negative bacteria. Sfh is a homologue of H-NS and is encoded by plasmid pSfR27. Sfh provides a 'stealth' function that allows pSfR27 to be transmitted to a new host without disrupting the competitive fitness of the new host We used ChIP-on-chip to profile Sfh (3xFLAG-tagged) and H-NS binding sites in Salmonella Typhimurium strain SL1344 and found that Sfh provides its 'stealth' function by targeting a sub-set of H-NS bound genes that display reduced levels of H-NS occupancy with the SL1344 chromosome upon acquisition of plasmid pSfR27

2009-12-05 | GSE19231 | GEO

Salmonella enterica serovar Typhimurium gene expression in exponential and stationary phase cultures

Project description:Transcriptional profiling of Salmonella Typhimurium strains SL1344 in exponential and stationary phase cultures

2017-03-29 | GSE97161 | GEO

Gene expression analysis of Salmonella enterica serovar Typhimurium strain SL1344 swap

Project description:We performed transcriptome abundance analysis of Salmonella Typhimurium strain SL1344 swap which has been genetically engineered to express the hns open-reading frame from the stpA promoter and the stpA open reading frame from the hns promoter. This strain is designated SL1344(swap). Transcript abundance was compared with that of wild-type SL1344. This comparison was performed to determine the effect of chromosome location of the expression of two related global regulators and how alterations to their expression patterns would impact on their regulons.

2013-11-09 | GSE52235 | GEO

Salmonella enterica serovar Typhimurium SL1344 cells: wildtype vs. ΔfabR mutant

Project description:Transcriptional profiling of Salmonella enterica serovar Typhimurium SL1344 cells comparing wildtype with ΔfabR mutant. Salmonella strains were cultured under free-living TSB conditions (TSB 1/20, 200 rpm, 25 °C) for 6h (ca. 2 x 108 cells/ml).

2014-12-31 | GSE52880 | GEO

Multi-omics Analysis of Salmonella enterica Serovar Typhimurium Infection of Wild Type C57BL/6 and Immunodeficient gp91-/-phox Mice

Project description:Three groups of sex- and aged-matched 9-12 week old C57BL/6 wild type mice and gp91-/-phox mice were infected by i.v. injection of bacterial suspensions: Group 1 represents wild type C57BL/6 mice infected with virulent S. Typhimurium SL1344 grown in vitro. (Raw files: OTNCS_AGSL1344#3C#9C#10C-XX) Group 2 represents wild type C57BL/6 mice infected with virulent S. Typhimurium SL1344 grown in vivo for 72 h in the Group 1 C57BL/6 mice. (Raw files: OTNCS_AGSL1344#9F#10F-XX) Group 3 represents immunodeficient gp91-/-phox mice infected with virulent S. Typhimurium SL1344 grown in vitro. (Raw files: OTNCS_AGSL1344#11C#12C#13C-XX) And Input (Raw files: OTNCS_AGSL1344mixedAa-XX and OTNCS_AGSL1344mixedAb-XX)

2018-10-26 | PXD005855 | Pride

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